天然产物研究与开发2017，Vol.29Issue(12)：2128-2133,2140,7.DOI:10.16333/j.1001-6880.2017.12.022
木犀草素通过抑制PTEN PI3K-AKT信号通路以及对免疫功能的调节作用介导对肺癌模型大鼠的干预研究
Effects of Luteolin on the Expression of PTEN PI3K-AKT Signaling Pathway and Immune Function in Lung Cancer Model Rats
摘要
Abstract
To investigate the effect of luteolin on PTEN-PI3K-AKT signal pathway and immune function in lung cancer model rats.The rats were given disposable infusion of carcinogenic iodized oil via left lobe bronchus to induce lung cancer model.The rats were treated with high,medium and low dose of luteolin and cyclophosphamide respectively for 16 weeks.The general condition,histopathology and lung index,spleen index were evaluated.The expression of EGF,TGF-β and VEGF was detected by enzyme-linked immunosorbent assay (ELISA).The CD3 +,CD4 +,CD8 +,CD4 +/CD8 + in T lymphocytes of rats were detected by flow cytometry.The expression of AKT-1,CyclinD1 and NF-κB mRNA in each group were detected by Real-time fluorescent quantitative PCR.The expression of PTEN,p-PI3K and p-AKT protein was detected by Western blot.The results showed that luteolin can significantly improve the pathology of lung tissue,decrease the lung index and increase spleen index.Luteolin cannot only reduce the levels of EGF,TGF-β,VEGF and AKT-1,CyclinD1,NF-κB mRNA and P-PI3K and p-AKT protein in lung tissue and the expression of CD8 + levels in blood,but also increase the expression of CD3 +,CD4 +,CD4 +/CD8 + levels in blood and PTEN protein in lung tissue.Luteolin can significantly improve the immune function and downregnlate the activity of PI3K/AKT signaling pathway in the Lung Cancer Model Rats.关键词
木犀草素/肺癌/肺指数/脾指数/PTEN-PI3K-AKTKey words
luteolin/lung cancer/lung index/spleen index/PTEN-PI3K-AKT分类
医药卫生引用本文复制引用
周亮,乔印玲,王莉,朱蕾,张建勇..木犀草素通过抑制PTEN PI3K-AKT信号通路以及对免疫功能的调节作用介导对肺癌模型大鼠的干预研究[J].天然产物研究与开发,2017,29(12):2128-2133,2140,7.基金项目
贵州省科技计划(黔科合LH字[2016]7473号) （黔科合LH字[2016]7473号）
