厦门大学学报（自然科学版）2018，Vol.57Issue(1)：58-64,7.DOI:10.6043/j.issn.0438-0479.201702038
鲍鱼内脏多糖的提取、纯化及其抗氧化和抑菌活性
Extraction,Purification and Antioxidant and Antibacterial Activity Analysis of Polysaccharides from Abalone Viscera
摘要
Abstract
The polysaccharides were obtained using abalone (Haliotis discus hannai Ino)viscera as the material in this study and enzymolyzed by alkaline protease,neutral protease,vernase,papain,pepsin and trypsin.The results of antioxidant experiments showed that the yield of polysaccharides was the highest up to 6.66％ when alkaline protease was used.Then through the single factor exper-iment and the orthogonal experiment,the optimal condition for alkaline protease extracting polysaccharides from abalone visceral was determined.Results of antioxidant activity showed that the concentration of primary polysaccharides from abalone viscera for 100％removal of hydroxyl free radical was 9.6 mg/mL,the concentration for 100％ removal of ABTS radical was 7.5 mg/mL,and the concentration of reducing capacity at A700=0.485 was 8.2 mg/mL.The results indicated that the polysaccharides had a good antiox-idant activity.Additionally,the polysaccharide content and antioxidant activity were the highest with secondary enzymolyzing by pep-sin.Furthermore,we got the high-purity abalone visceral polysaccharides with Sevage method and repeated freezing and thawing method to remove proteins,and with hydrogen peroxide method for depigment.Finally,double plate method was used to measure an-tibacterial activity of the abalone visceral polysaccharides,which showed that the purified polysaccharides suppressed reproduction of Staphylococcus aureus,Salmonella typhimurium,Escherichia coli and Pseudomonas aeruginosa efficently.关键词
鲍鱼内脏/多糖/提取/纯化/抗氧化活性/抑菌活性Key words
abalone viscera/polysaccharide/extraction/purification/antioxidant activity/antibacterial activity分类
生物科学引用本文复制引用
张瑞娟,柯莉娜,郑静,石艳,王勤..鲍鱼内脏多糖的提取、纯化及其抗氧化和抑菌活性[J].厦门大学学报（自然科学版）,2018,57(1):58-64,7.基金项目
国家海洋公益性行业科研专项(201405016) （201405016）
