厦门大学学报（自然科学版）2018，Vol.57Issue(1)：130-136,7.DOI:10.6043/j.issn.0438-0479.201705046
对流实时荧光定量PCR系统设计
Design on Real-time Fluorescence Quantitative PCR System Based on Natural Convection
翁振宇 1闵小平 2王海 3卓之豪 2葛胜祥3
作者信息
- 1. 厦门大学公共卫生学院,福建 厦门 361102
- 2. 厦门大学信息科学与技术学院,福建 厦门 361005
- 3. 国家传染病诊断试剂与疫苗工程技术研究中心(厦门大学),福建 厦门 361102
- 折叠
摘要
Abstract
A PCR system with real-time fluorescent quantitative technology and natural convection technology was designed.This paper mainly introduced the two-channels structure and the work principle of fluorescence excitation and fluorescence detection.In the system,fiber optics were used as an optical transmission medium and a charge coupled device(CCD)camera was applied to collect the change of fluorescent signal.To ensure that the system work steadily,the circuit and software were designed independently.Final-ly,we showed the system's potential of rapid nucleic acid amplification and real-time quantitation by amplifying gradient-diluted gene template of cytomegalovirus(CMV).关键词
实时荧光定量PCR/自然对流PCR/双通道/快速扩增Key words
real-time fluorescence quantitative PCR/natural convection PCR/two-channel structure/rapid amplification分类
机械制造引用本文复制引用
翁振宇,闵小平,王海,卓之豪,葛胜祥..对流实时荧光定量PCR系统设计[J].厦门大学学报（自然科学版）,2018,57(1):130-136,7.
