医药导报2018，Vol.37Issue(2)：236-238,3.DOI:10.3870/j.issn.1004-0781.2018.02.023
高效液相色谱法同时测定
复方苯甲酸涂剂中苯甲酸与水杨酸含量
Simultaneous Determination of Benzoic Acid and Salicylic Acid in Compound Benzoic Acid Embrocation by High Performance Liquid Chromatography
徐硕 1徐文峰 1吴学军 1金鹏飞1
作者信息
- 1. 北京医院药学部,国家老年医学中心,北京 100730
- 折叠
摘要
Abstract
Objective To develope a high performance liquid chromatography ( HPLC) method for simultaneous determination of benzoic acid and salicylic acid in compound benzoic acid embrocation. Methods An Alltima C18 column (150 mm×4.6 mm,5 μm) was used for the separation, with methanol-0.1％ phosphoric acid (50:50) as the mobile phase at the flow rate of 1.0 mL?min-1 .The detection wavelength was set at 236 nm.The column temperature was 35 ℃ . Results The good linearity was obtained with the correlation coefficient (r) of 1.0000 for benzoic acid and salicylic acid;the precisions and stability were satisfactory, and the relative standard deviations (RSDs) were less than 0.3％.The average recoveries (n= 9) were 100.15％ and 99.85％ for benzoic acid and salicylic acid, respectively. Conclusion The established method is accurate, simple and rapid, and can be utilized for the simultaneous determination of benzoic acid and salicylic acid in compound benzoic acid embrocation.关键词
苯甲酸涂剂,复方/苯甲酸/水杨酸/色谱法,高效液相Key words
Benzoic acid embrocation,compound/Benzoic acid/Salicylic acid/Chromatography,high-performance liquid分类
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徐硕,徐文峰,吴学军,金鹏飞..高效液相色谱法同时测定
复方苯甲酸涂剂中苯甲酸与水杨酸含量[J].医药导报,2018,37(2):236-238,3.
