渭北旱地苹果园生草覆盖下不同肥料配施对土壤养分和酶活性的影响
Effects of grass cover combined with different fertilization regimes on soil nutrients and enzyme activities in apple orchard in Weibei dryland, China
摘要
Abstract
The split-plot design was adopted in this experiment,with main treatments of grass cover and control and sub-treatments included four fertilization regimes:no fertilization,CK;manure,M;N,P and K fertilizer,NPK;and NPK fertilizer combined with manure,MNPK.Microplate fluofimetry was used to study the effects of grass cover combined with different fertilization regimes on the enzyme activities in apple orchard.The results showed that after mowing the grass (the residues were left on the soil surface as mulch),the soil water content,available P,nitrite nitrogen and the activities of βX,NAG,βG,CBH were increased compared to the control,with no significant differences for total nitrogen,soil organic carbon,and AKP activity.For grass cover treatment,the total nitrogen,soil organic carbon,and the activities of βX,NAG,βG,CBH,AKP were both improved before and after mowing the grass.However,the soil water content,available phosphorus,and nitrate nitrogen of grass cover treatment were lower than that of the control before mowing the grass.Under grass cover condition,the total nitrogen,available P,and soil organic carbon of M and MNPK were higher than that of CK and NPK in both before and after mowing the grass periods,with the activities of βX,NAG,βG,CBH,AKP of MNPK higher than that of NPK.Under the control condition,the available P,soil organic carbon (SOC),nitrite nitrogen,total nitrogen and the activities of βG,CBH,AKP of MNPK higher than that of CK and NPK before and after mowing the grass.Redundancy analysis showed that the activities of soil enzymes were significantly correlated with the soil nutrients,and could reflect the soil fertility.Thus,grass cover combined with MNPK significantly increased the soil nutrient contents and soil enzyme activities,and was an important practice to prevent the decrease of soil fertility and benefit the sustainability of local apple industry.关键词
土壤酶活性/微孔板荧光法/冗余分析/土壤质量Key words
soil enzyme activity/microplate fluorimetric assay/redundancy analysis/soil quality引用本文复制引用
巩庆利,翟丙年,郑伟,刘杰,郑朝霞,赵志远,李紫燕,王朝辉..渭北旱地苹果园生草覆盖下不同肥料配施对土壤养分和酶活性的影响[J].应用生态学报,2018,29(1):205-212,8.基金项目
本文由公益性行业(农业)科研专项(201303104,201103005-9)、陕西省农业科技创新转化项目(NYKJ-2015-17)、陕西省科技统筹创新工程计划项目(2011KTZB02-02-05)、杨凌示范区农业科技示范推广能力提升项目(2015-TS-18)、西北农林科技大学试验示范基地科技成果推广项目(TGZX2014-16,TGZX2015-40)和杨凌示范区科技计划项目(2015NY-05)资助 This work was supported by the Special Fund for Agro-scientific Research in the Public Interest of China (201303104,201103005-9),the Agriculture Science Technology Achievement Transformation Fund of Shaanxi Province (NYKJ-2015-17),the Science and Technology Innovation Project of Shaanxi Province (2011KTZB02-02-05),the Project of Promoting Agricultural Science and Technology Demonstration of Yangling (2015-TS-18),the Scientific and Technological Achievements Promotion Project of Northwest A&F University (TGZX2014-16,TGZX2015-40) and the Project of Science and Technology Demonstration of Yangling (2015NY-05). (农业)