针刺研究2018，Vol.43Issue(1)：1-7,7.DOI:10.13702/j.1000-0607.170181
电针、艾灸预处理对心肌缺血再灌注损伤大鼠心肌自噬相关蛋白LC3、Beclin1表达的影响
Effect of Electroacupuncture and Moxibustion Pretreatment on Expression of Autophagy Related Proteins LC 3 and Beclin 1 in Rats with Myocardial Ischemia-reperfusion Injury
摘要
Abstract
Objective To observe the effect of electroacupuncture (EA) and moxibustion (Moxi) pretreatment on myocardial pathological and structural changes and expression of autophagy related protein LC 3 Ⅰ / Ⅱ and Beclin 1 in rats with myocardial ischemia-reperfusion injury (MI/RI),so as to explore their mechanisms underlying improving MI/RI.Methods Forty SD rats were randomly divided into sham operation,model,ischemic preconditioning (IP),EA and Moxi groups (n =8 in each group).EA (10 Hz/50 Hz,1 mA) or Moxi (ignited moxa stick) was respectively applied to bilateral "Neiguan"(PC 6) for 20 min,once daily for 7 days.The MI/RI model was established by occlusion of the anterior descending branch of the left coronary artery for 40 min,followed by reperfusion for 60 min.The left ventricular (LV) tissue samples were collected and analyzed for pathological (H.E.staining) and ultrastructural changes,for myocardial apoptosis (apoptotic index=number of apoptotic cells/total number of cardiomyocytes × 100 ％) with terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) method,and for the expression of LC 3 and Beclin 1 in myocardial cells with Western blot.Results Following MI/RI,H.E.staining revealed a disorder of arrangement of cardiomyocytes with vague border,inflammatory cell infiltration,intracellular swelling with bleeding,necrosis and dissolution of partial striated muscles of the left ventricle under light microscope,and dual staining of Uranyl acetate and leadnitrate showed atrophy,arrangement disorder,dissolution,necrosis,and interstitial edema of partial myocardial fibers,mitochondrial structural disorder,vacolation,and large body of autophagosomes with bilayers,etc.in ultrastructure,which was relatively lighter in both EA and Moxi groups.The apoptosis index,expression levels of myocardial LC 3 Ⅱ and Beclin 1 and the ratio of LC 3 Ⅱ/LC 3 Ⅰ were significantly higher in the model group than those in the sham operation group (P＜0.01),but the expression level of LC 3 Ⅰ was considerably down-regulated in the model group relevant to the sham operation group (P＜0.01).Following the intervention and MI preconditioning,the increased apoptosis index and expression levels of LC 3 Ⅱ and Beclin 1 proteins and the ratio of LC 3 Ⅱ/LC 3 Ⅰ were obviously down-regulated in the IP,EA and Moxi groups relevant to the model group (P＜0.01),and the decreased expression of LC 3 Ⅰ protein was up-regulated obviously in the 3 treatment groups (P＜0.05,P＜0.01).The effects of EA were significantly superior to those of IP and Moxi groups in down-regulating apoptosis index and expression of LC 3 Ⅱ and Beclin 1 and the ratio of LC 3 Ⅱ/LC 3 Ⅰ and in up-regulating expression of LC 3 Ⅰ (P＜0.05,P＜0.01).Conclu-sion Both EA and Moxi preconditioning of PC 6 have a protective effect on ischemic myocardium in MI/RI rats,which is probably related to their effects in regulating expression of myocardial autophagy proteins as LC 3 Ⅰ / Ⅱ and Beclin 1.关键词
电针预处理/艾灸预处理/心肌缺血再灌注/缺血预适应/心肌自噬相关蛋白Key words
Electroacupuncture pretreatment/Moxibustion pretreatment/Myocardial ischemia-reperfusion/Ischemic preconditioning/Myocardial autophagy proteins分类
医药卫生引用本文复制引用
谭成富,陈美琳,李姣兰,王超,杜琳,刘薇薇,宋瑾,冯果,严洁,阳晶晶,唐雅妮..电针、艾灸预处理对心肌缺血再灌注损伤大鼠心肌自噬相关蛋白LC3、Beclin1表达的影响[J].针刺研究,2018,43(1):1-7,7.基金项目
国家自然科学基金项目(No.81574080) （No.81574080）
湖南省高校创新平台开放基金项目(No.15 k 093) （No.15 k 093）
湖南省中医管理局课题(No.201521) （No.201521）
针灸推拿省级重点学科开放基金一般项目 （）
