针刺研究2018，Vol.43Issue(1)：8-13,6.DOI:10.13702/j.1000-0607.170517
电针对高脂诱导胰岛素抵抗大鼠肝脏固醇调节元件结合蛋白-1c、脂肪酸合成酶的影响
Effect of Eletroacupuncture Intervention on Insulin Resistance, Lipid Metabolic Disorder and Expression of Hepatic SREBP-1 c and Fatty Acid Synthase Proteins in Rats with Hyperlipidemia
摘要
Abstract
Objective To observe the effect of electroacupuncture(EA) of "Fenglong" (ST 40) and "Sanyinjiao" (SP 6) on lipid metabolic disorder,insulin resistance (IR) and expression of sterol regulatory element blinding protein-1 (SREBP-1) c and fatty acid synthase (FAS) proteins in the liver tissue in hyperlipidemia rats with IR,so as to reveal its mechanisms underlying improvement of IR.Methods Forty male SD rats were randomly divided into blank control,model,medication and EA groups (n =8 in each group).The IR model was established by feeding the rat with high-fat diet.Rats of the medication group were treated by intragastric administration of pioglitazone (10 mL/kg).For rats of the EA group,EA (2 Hz/100 Hz,1 mA) was applied to bilateral ST 40 and SP 6,once daily for 14 days.The insulin sensitivity index (ISI) was assessed by calculating 60-120 min glucose infusion rate (GIR 60-120) with euglycemic hyperinsulinemic clamp in reference to Kraegen's and colleagues' methods.Fasting blood samples (10 mL) were collected and analyzed for fasting blood glucose (FBG) using enzyme method,serum fasting insulin(FINS) using ELISA,free fatty acid(FFA) using spectrophotometry,and total triglyceride(TG) and total cholesterol(TC)employing glycerine phosphate oxidase peroxidase (GPO-PAP) assay,low density lipoprotein(LDL),high density lipoprotein (HDL) levels using combined filiter paper activity and lipase activity methods,respectively.The IR level was assessed by calculating homeostatic model assessment of insulin resistance (HOMA-IR) using the formula (FBG × FINS)/22.5.The expression levels of SREBP-1 c and FAS proteins in the liver tissue were detected by Western blot.Results Following modeling,the GIR 60-120 and serum HDL were significantly decreased(P＜0.01),and the HOMA-IR,serum FBG,FINS,FFA,TG,TC and LDL,and the expression levels of hepatic SREBP-1 c and FAS proteins were significantly increased in comparison with the blank control group(P＜0.01).After the intervention,the decreased GIR 60-120 and serum HDL levels were considerably up-regulated (P＜0.01),and the increased FBG,FINS,FFA,TG,TC and LDL,and hepatic SREBP-1 c and FAS protein levels were notably down-regulated in both EA and medication groups relevant to the model group (P＜0.05,P＜0.01).No significant differences were found between the EA and medication groups in all the indexes mentioned above (P＞0.05).Conclusion EA intervention is able to improve the disorder of lipid metabolism of IR rats,which may be associated with its effects in reducing the expression of SREBP-1 c and FAS proteins and in lowering the synthesis of fatty acid.关键词
电针/高脂血症/胰岛素抵抗/脂质代谢紊乱/固醇调节元件结合蛋白/脂肪酸合成酶Key words
Electroacupuncture/Hyperlipidemia/Insulin resistance/Disorder of lipid metabolism/Hepatic sterol regulatory element blinding protein-1 (SREBP-1)c/Fatty acid synthase (FAS)分类
医药卫生引用本文复制引用
李知行,张海华,蓝丹纯,张弘弢,陈小砖,孙健..电针对高脂诱导胰岛素抵抗大鼠肝脏固醇调节元件结合蛋白-1c、脂肪酸合成酶的影响[J].针刺研究,2018,43(1):8-13,6.基金项目
国家自然科学基金项目(No.81774394、81303042、81273845) （No.81774394、81303042、81273845）
深圳市卫生计生系统科研项目博士创新项目(No.SZBC 2017025) （No.SZBC 2017025）
