针刺研究2018，Vol.43Issue(1)：14-19,6.DOI:10.13702/j.1000-0607.170302
电针对痛觉敏化大鼠背根神经节蛋白酶激活受体2的影响
Effect of Electroacupuncture on Pain Reaction and Content of Proteinase-activated Receptors 2 in Dorsal Root Ganglion in Hyperalgesia Rats
摘要
Abstract
Objective To observe the effect of electroacupuncture (EA) on mechanical hyperalgesia threshold (MHTs)and thermal hyperalgesia threshold (THTs) and content of proteinase-activated receptors 2 (PAR 2) in dorsal root ganglia (DRG) in rats with inflammatory pain,so as to explore its peripheral mechanism underlying improvement of inflammatory pain.Methods The present study contains two parts.1) In the first part,27 male SD rats were randomized into sham hyperalgesic priming (shamHP) group and real hyperalgesic priming (HP) group (n =5 in the sham-HP group and n=6 in the HP group for the test of MHTs,n =8 in the two groups for the test of THTs).The sham-HP model was established by subcutaneous injection of normal saline into the left plantar part of the hind-paw,and the HP model established by subcutaneous injection of 1％ carragenan (the first injection)into the same left hind paw,followed by injection of PGE2 (100 ng/25 μL,the second injection) into the dorsum pedis of the same hind paw 7 days after the first injection.The ipsilateral paw withdrawal latencies (MHTs and THTs) were detected before and 5 h,3 d and 6 d after the first injection,0.5,4 and 24 h after the second injection.2) In the second part,64 male SD rats were randomly divided into sham-HP,HP,sham-EA and EA groups (n =16 in each group).The sham-HP and HP models were made in the same way as the first part.Both"Zusanli"(ST 36)and "Kunlun"(BL 60) were punctured with filiform needles in the sham-EA group and also stimulated with EA:2 Hz/100 Hz,0.5-1.5 mA (0.5 mA increase per 10 min) for 30 min in the EA group,1 time/d for 7 d.Both ipsilateral MHTs and THTs were observed at the same time-points of the first part and the PAR 2 protein content in the L 4-L 6 DRGs was assayed by ELISA 24 h after the second injection.Results 1) In the first part of the study,compared with the sham-HP group,the MHTs at 5 h and 3 d,and THT at 5 h after the first injection,and MHTs,and THTs at 4 and 24 h after the second injection were significantly decreased in the HP group (P＜0.01,P＜0.05).2) In the second part of the study,compared with the HP group,the MHTs at 4 and 24 h after the second injection and the THTs at 3 d after the first injection,4 and 24 h after the second injection were significantly up-regulated in the EA group (P＜0.01,P＜0.05).The content of PAR 2 in the DRGs (L 4-L 6) was significantly higher in the HP group than in the sham-HP group (P＜0.05),but considerably lower in the EA group than in the HP group (P＜0.05).Conclusion EA can suppress hyperalgesia priming in inflammatory pain rats which may be related to its effect in down-regulating PAR 2 level in the lumbar DRGs.关键词
电针/炎性痛/痛觉敏化诱发/痛转化/蛋白酶激活受体2/腰背根神经节Key words
Electroacupuncture/Inflammatory pain/Hyperalgesic priming/Pain transformation/Proteinase-activated receptors 2/Lumbar dorsal root ganglia分类
医药卫生引用本文复制引用
胡海宇,丁家威,吴叶琪,项亚楠,方剑乔,宣丽华,杜俊英,房军帆..电针对痛觉敏化大鼠背根神经节蛋白酶激活受体2的影响[J].针刺研究,2018,43(1):14-19,6.基金项目
国家自然科学基金青年项目(No.81603692、81603690) （No.81603692、81603690）
浙江省科技厅公益性(实验动物平台)项目(No.2016 C 37135) （实验动物平台）
2016年浙江省大学生科技创新活动暨新苗人才计划资助项目(No.2016 R 410005) （No.2016 R 410005）
浙江省一流学科(中医学)资助(浙政办函[2016]6号) （中医学）
