针刺研究2018，Vol.43Issue(1)：34-37,4.DOI:10.13702/j.1000-0607.170064
温通针法对过敏性鼻炎大鼠血清免疫球蛋白E、白介素-1β、肿瘤坏死因子-α含量的影响
Effect of Warm Acupuncture on the Levels of Serum Immunoglobulin E, Interleukin-1 β and Tumor Necrosis Factor-α in Rats with Allergic Rhinitis
摘要
Abstract
Objective To observe the effect of warm acupuncture on behavior and contents of serum immunoglobulin E (IgE),interleukin-1 β(IL-1 β) and tumor necrosis factor-α(TNF-α) in allergic rhinitis(AR) rats,so as to explore its mechanism underlying improving AR.Methods Forty Wistar rats were randomly divided into four groups:control group,model group,medication group and warm acupuncture group(10 rats/group).The AR model was established by intraperitoneal injection of sensitization and nasal drip.The rats in the medication group were given fluticasone propionate nasal spray,daily for 10 days.Warm acupuncture was applied to "Fengchi"(GB 20),"Yintang"(GV 29),"Yingxiang"(LI 20) for 60 seconds,once daily for 10 days.Behavioral scores were used to evaluate behavioral changes in rats.Enzyme linked immunosorbent assay (ELISA) was used to detect the expression levels of serum IgE,IL-1 β and TNF-α.Results Behavioral scores of the model group were significantly higher than those of the control group 0,3,7 and 10 days after modeling (P＜0.05).After treatment,the behavioral scores of medication group and the warm acupuncture group were lower than those of the model group (P＜0.05),and the score was more lower in the warm acupuncture group than in the medication group (P＜0.05).Compared with the control group,the levels of serum IgE,IL-1 β and TNF-α in the model group were all increased (P＜0.01),while the levels of serum IgE,IL-1 β and TNF-α were decreased in the medication and warm acupuncture groups after treatment in comparison with the model group (P＜0.05,P＜0.01).Compared with the medication group,the levels of serum IgE,IL-1 β and TNF-α were significantly lower in the warm acupuncture group (P＜0.05,P＜0.01).Conclusion The expression levels of IgE,IL-1 β and TNF-α were elevated in serum after AR attack.Warm acupuncture can improve the symptoms of AR rats,which may be associated to its effect in inhibiting the expression of serum IgE,IL-1 β and TNF-α.关键词
温通针法/过敏性鼻炎/行为学/免疫球蛋白EKey words
Warm acupuncture/Allergic rhinitis/Behavior/Immunoglobulin E分类
医药卫生引用本文复制引用
郑先丽,田永萍,雒海燕,赵耀东,刘翔毅,姜影,马承旭,王明娟,刘敏..温通针法对过敏性鼻炎大鼠血清免疫球蛋白E、白介素-1β、肿瘤坏死因子-α含量的影响[J].针刺研究,2018,43(1):34-37,4.基金项目
甘肃郑氏针法学术流派传承工作室开放基金(No.LP 0128060-KFJJ 05) （No.LP 0128060-KFJJ 05）
甘肃省科技支撑计划项目(No.1304 FKCA 096) （No.1304 FKCA 096）
甘肃省自然科学基金项目(No.096 RJZA 043) （No.096 RJZA 043）
甘肃省重点中医药科研项目(No.GZK-2010-Z 12) （No.GZK-2010-Z 12）
