中风与神经疾病杂志2018，Vol.35Issue(1)：4-9,6.
SB-271046对匹罗卡品致痫大鼠空间学习记忆的影响
Effects of SB-271046 on spatial learning-memory in pilocarpine-induced epileptic rats
摘要
Abstract
Objective Our primary objective is to investigate the effects of 5-HT6 receptor antagonist SB-271046 on spatial learning-memory in pilocarpine-induced epileptic rats. Methods 50 male adult Sprague-Dawley rats were divided into 2 groups,vehicle control group (n=10) and pilocarpine(PILO) treated group (n=50). Status epilepticus (SE) of rats was induced by intraperitoneal ( i. p. ) injection of LiCl-pilocarpine. According to Racine classify,seizures up to IV and last more than 30mins are supposed to be SE. Rats with successful SE were then divided into 3groups:mode control group (HP-CD,lateral ventricle (LV)injection with HP-β-CD,4 μl),10 μg-SB271046 group(10 μg-SB,LV injection with SB-271046,4μl),20μg-SB271046 group(20μg-SB,LV injection with SB-271046,4μl). Unsuccessful SE rats and died rats were both excluded from our experiment. Spatial learning-memory was evaluated to all groups by Y-maze and Morris water maze after 6 weeks. Results In Y-maze spontaneous alternation behavior test(SAB),We found significant decreasing of alternation rate in HP-CD group(P<0. 01). Besides,we saw reversion of alternation rate in the rats in SB-271046 injected groups,especially 20 μg-SB271046 group(P<0. 05). In Y-maze novel object discrimination 4h ITI test(NOD),new arm percentage of HP-CD also decreased significantly(P<0. 01),but SB-271046 didn't reverse this defect. In Morris water maze acquisition stage,the latency of different groups were gradually shorten from day 1 to day 5;meanwhile,the mean la-tency of the rats in HP-CD was prolonged significantly(P<0. 01),the most obvious improvement also was found in 20 μg-SB271046 group(P<0. 05). In retention stage,the results of 3 items: means latency,numbers of crossing target and per-centage of performance time in target zone indicated the memory of rats in HP-CD group were damaged,SB-271046 didn' t reverse the defects. Conclusion 5-HT6 receptor antagonist SB-271046 can reverse epileptic rats' spatial learning-memory defect,especially in acquisition stage,and maybe have dose-effect relationship.关键词
癫痫/5-HT6受体/SB-271046/Morris水迷宫/Y迷宫Key words
Epilepsy/5-HT6 receptor/SB-271046/Morris water maze/Y-maze分类
医药卫生引用本文复制引用
黄明珠,黄华品,林婉挥,陈圣根..SB-271046对匹罗卡品致痫大鼠空间学习记忆的影响[J].中风与神经疾病杂志,2018,35(1):4-9,6.基金项目
国家自然科学基金(面上项目)资助(No.81371426) （面上项目）
