中国农业大学学报2018，Vol.23Issue(2)：36-42,7.DOI:10.11841/j.issn.1007-4333.2018.02.05
高效降胆固醇植物乳杆菌的筛选及其益生潜能初探
Screening and evaluation of cholesterol-lowering Lactobacillus plantarum strains
摘要
Abstract
The aim of this study was to screen the Lactobacillus with efficient cholesterol-lowering ability and discover its beneficial potency.Eleven Lactobacillus strains were isolated from homemade pickle samples and screened for high cholesterol-lowering by phthalic aldehyde method and 16S rDNA molecular biology identification.The acid tolerance,bile tolerance and antibacterial tests were then conducted to analyze their probiotic properties.The results showed that:1) High cholesterol-lowering strains,PL2,PL5,PL16 and PL19,were obtained from the screen,and their cholesterol degradation rate were 48.22 ％,51.67 ％,48.43 ％ and 48.56 ％,respectively.2) The four strains were identified as L.plantarum by morphological observation and molecular biology identification.3) All strains survived in pH 2.0 for three hours.Strains PL2 and PL5 survived under ambient of 0.3％ bile salt concentration for three hours.4) It was found that strain PL5 displayed significant inhibitory effect on four common pathogens.In conclusion,strain PL5 was a potential probiotics with excellent cholesterol-lowering effect.关键词
植物乳杆菌/降胆固醇/耐酸/耐胆盐/抑菌作用Key words
Lactobacillus plantarum/cholesterol-lowering/acid tolerance/bile salt tolerance/antibacterial effect分类
农业科技引用本文复制引用
周海柱,高云航,郭玮,勾长龙,陶大鹏,娄玉杰..高效降胆固醇植物乳杆菌的筛选及其益生潜能初探[J].中国农业大学学报,2018,23(2):36-42,7.基金项目
国家重点研发计划(2016YFD0501409-03) （2016YFD0501409-03）
国家自然基金项目(NSFC:31372331) （NSFC:31372331）
现代农业产业技术体系建设专项(CARS-38) （CARS-38）
公益性行业(农业)科研专项(201303091) （农业）
