中国农业科学2018，Vol.51Issue(1)：96-104,9.DOI:10.3864/j.issn.0578-1752.2018.01.009
灵芝多糖拌种对小麦抗纹枯病的诱导效应及生长发育影响
Induction Effect of Sharp Eyespot of Wheat and the Effect of Wheat Growth After Ganoderma lucidum Polysaccharides (GLP) Seed Dressing
摘要
Abstract
[Objective] Fungal polysaccharides can regulate plant growth,activate plant defense responses,and induce plant resistance to plant fungal and viral diseases.The objective of this study is to investigate the induction effect of sharp eyespot of wheat and the effect on wheat growth after Ganoderma lucidum polysaccharides (GLP) seed dressing.[Method] Three wheat cultivars including highly susceptible cuttivar Jimai 22,middle susceptible cultivar Shannong 23 and resistant cultivar Luyuan 502 were chosen to test.Using the pot culture method,each pot was added with 20 mL of pathogenic fungi suspension,seeds were treated with the GLP,the control group was treated with water.The disease index and disease reduction against sharp eyespot of wheat were investigated.The mechanism of induced resistance in terms of defense enzyme activity,chlorophyll and malondialdehyde (MDA) content were evaluated.At the same time,the germination rate,plant height and root vigor were determined.[Result] After GLP seed dressing,Jimai 22,Shannong 23,Luyuan 502 cultivars had the same rule on sharp eyespot of wheat.GLP seed dressing promoted the growth of wheat,4,8 g a.i./100 kg seed dose of GLP of Jimai 22 seed germinating potential were 53.50％ and 52.75％,significantly higher than that of the control.After 7 d of wheat emergence,the plant height of wheat treated with GLP was significantly higher than that of the control group,and the higher the treatment dose,the more obvious the effect of induction enhancement.GLP seed dressing could significantly improve the root activity and the chlorophyll content of wheat,the root activity of 8 g a.i./100 kg seed dose of GLP of Jimai 22 was 0.26 mg·g1·h-1,which was 2.77 times than that of the control.The chlorophyll a and b contents of 8 g a.i./100 kg seed dose of GLP were 1.96,0.96 mg·g-1,respectively,which was 1.52,1.44 times than that of thecontrol.GLP seed dressing could produce certain effect on prevention and control of sharp eyespot of wheat.Take the highly susceptible cultivar Jimai 22 as an example,8 g a.i./100 kg seed dose of GLP in wheat seed dressing,the control effects on sharp eyespot of wheat were 33.7％,31.9％ and 30.4％ on the 7,14 and 21 d after wheat completely germination.At the same time,the protective enzyme activity of PAL,POD,SOD of leaves increased obviously,while the content of MDA decreased.Eight g a.i./100 kg seed dose of GLP by seed dressing in Jimai 22,the activity of PAL,SOD and POD was 60.72 U·min-1·g-1 FW,2.45 U·g-1 FW and 135.67 U·min-1·g-1 FW,respectively,which was 1.10,1.32 and 1.13 times than that of the control.The content of MDA was 1.48 μmol·g-1,which was 26.1％ lower than that of the control group.[Conclusion] The wheat seed dressed with GLP can promote the germination of wheat seeds and the growth of wheat seedlings,increase the content of chlorophyll.At the same time,it can also induce wheat sheath blight disease resistance,increase the activity of defense enzymes and decrease the content of MDA in wheat leaves.关键词
灵芝多糖/小麦纹枯病/诱导抗性/防御酶Key words
Ganoderma lucidum polysaccharides/sharp eyespot of wheat/induced resistance/defensive enzymes引用本文复制引用
张中霄,王红艳,王开运,王东,姜莉莉..灵芝多糖拌种对小麦抗纹枯病的诱导效应及生长发育影响[J].中国农业科学,2018,51(1):96-104,9.基金项目
国家公益性行业(农业)科研专项(201503130) （农业）
