中国全科医学2018，Vol.21Issue(3)：334-337,4.DOI:10.3969/j.issn.1007-9572.2017.00.247
左归丸对少弱精子症模型大鼠睾丸组织干细胞因子及其mRNA表达的影响研究
Effects of Zuogui Pills on the Expression Levels of Stem Cell Factor Protein and mRNA in the Testis of Oligoasthenozoospermia Rats Induced by Tripterygium Glycosides
摘要
Abstract
Objective To observe the effects of Zuogui pills on the expression levels of stem cell factor (SCF) protein and mRNA in the testis of oligoasthenozoospermia rats induced by tripterygium glycosides (TG), and based on this, to explore the mechanism of Zuogui pills on improving the reproductive function of these rats.Methods From March to April 2014, 36 SD, SPF grade rats were randomly divided into normal group, TG model group and Zuogui pill treatment group, with 12 rats in each. The normal group received intragastric administration of deionized water during the whole intervention period; for modelling, at 4 weeks before the experiment, both TG model group and Zuogui pill treatment group received oral administration of the suspension of TG 40 mg? kg-1? d-1, then, during the 5th-8th weeks of experiment, the TG model group received intragastric administration of deionized water, Zuogui pill treatment group received oral administration of the suspension of Zuogui pill 6 g? kg-1? d-1. At the end of the 8-week intervention, all the rats were sacrificed, and their testicular tissues were collected aseptically, fixed, embedded and sectioned. Immunohistochemistry was used to detect the expression level of SCF protein in the testicular tissue. Real-time PCR was employed to measure the expression level of SCF mRNA in the testicular tissue.Results Both the expression levels of SCF protein and mRNA in the testicular tissue were significantly higher compared with the TG model group (P<0.05).Conclusion Zuogugui pill can promote the division and proliferation of spermatogonial stem cells and inhibit the apoptosis of spermatogenic cells by up regulating the expression levels of SCF protein and mRNA.关键词
弱精子症/少精子症/左归丸/大鼠Key words
Asthenozoospermia/Oligospermia/Zuogui pill/Rats分类
医药卫生引用本文复制引用
王彬,李霄,马凰富,祝雨田,马健雄,王继升,党进,李海松..左归丸对少弱精子症模型大鼠睾丸组织干细胞因子及其mRNA表达的影响研究[J].中国全科医学,2018,21(3):334-337,4.基金项目
国家自然科学基金资助项目(81273756) （81273756）
