中国人兽共患病学报2017，Vol.33Issue(12)：1089-1093,5.DOI:10.3969/j.issn.1002-2694.2017.12.007
江西省汉滩型汉坦病毒分离株AYW89-15全基因组序列分析
Complete genome sequence analysis of Hantaan virus strain AYW89-15 isolated in Jiangxi, China
摘要
Abstract
We investigated the complete genome characteristic of the Hantaan virus strain AYW89-15 isolated in Jiangxi Province,China.Primers were designed and were used to amplify the complete S,M and L segments by RT-PCR.The PCR product were then cloned and sequenced,the gene sequences were analyzed with DNAStar and MEGA6.0 software.Result showed that the complete gene sequence was 11 848 nucleotides in length,the S,M and L segments were 1 699 nt,3 616 nt and 6 533 nt respectively,encoding 429,1 133 and 2 151 amino acids respectively.The sequence identities between stain AYW89-15 and other Hantaan virus were 79.7％-87.3％ at the nucleotide level and 92.3％-98.4％ at the amino acid level.Phylogenetic analysis of HV showed AYW89-15 belonged to a new HTNV lineage.AYW89-15 was a new subtype of HTNV that exists in Jiangxi Province.关键词
汉滩型汉坦病毒/全基因组/基因分型Key words
Hantaan virus/complete gene sequence/genotype分类
医药卫生引用本文复制引用
刘师文,熊英,龚甜,徐刚,施勇,李健雄,张艳妮,周珺,刘晓庆,肖芳..江西省汉滩型汉坦病毒分离株AYW89-15全基因组序列分析[J].中国人兽共患病学报,2017,33(12):1089-1093,5.基金项目
江西省卫生厅科技计划项目(No.20123156)Supported by the Provincial Health Department of Jiangxi (No.20123156) （No.20123156）
