中国人兽共患病学报2017，Vol.33Issue(12)：1098-1102,1109,6.DOI:10.3969/j.issn.1002-2694.2017.12.009
深圳地区甲氧西林耐药金黄色葡萄球菌耐药性及分子流行病学特征
Antibiotic resistance and molecular epidemiology characteristics of methicillin-resistant Staphylococcus aureus in Shenzhen area
摘要
Abstract
The aim of this study is to investigate antibiotic resistance and molecular epidemiology characteristics of methicillin-resistant Staphylococcus aureus (MRSA) in Shenzhen area.We collected 428 Staphylococcus aureus isolates from eight hospitals in Shenzhen in 2012.According to the results of minimum inhibitory concentration (MIC) to cefoxitin,26.2％ of Staphylococcus aureus isolates (112/428) were identified as MRSA.The MIC of 10 antimicrobial agents was determined by agar dilution method.Panton-Valentine leucocidin(PVL) was detected by polymerase chain reaction(PCR).Positive strains of PVL were detected by multilocus sequence typing(MLST).Among the 112 strains,the resistance rates to trimethoprim-sulfamethoxazole,moxifloxacin,ciprofloxacin,gentamicin,erythromycin,clindamycin and tetracycline were 4.46％,12.50％,16.96％,19.64％,46.42％,25.00％ and 26.79％ respectively.No isolates resistant to vancomycin,linezolid and teicoplanin were found.Among the 112 strains,there were 13 (11.61％) strains carried PVL gene.There were no significant differences in the resistance rates of PVL positive strains and negative strains to 10 kinds of antimicrobial agents.Among the 13 strains carried PVL gene,7 kinds of old sequences and 1 kind of new sequence type were found by MLST.ST338 and ST25 were the most common type.All the data indicate that surveillance of MRSA in Shenzhen has a distinct genetic background from other regions.关键词
甲氧西林耐药金黄色葡萄球菌/琼脂稀释法/耐药性/杀白细胞毒素基因/多位点序列分型Key words
methicillin-resistant Staphylococcus aureus/agar dilution/antibiotics resistance/Panton-Valentine leucocidin/multilocus sequence typing分类
医药卫生引用本文复制引用
李文青,程锦娥,吴伟元,周小海,卢月梅,吴文苑,龚文波..深圳地区甲氧西林耐药金黄色葡萄球菌耐药性及分子流行病学特征[J].中国人兽共患病学报,2017,33(12):1098-1102,1109,6.基金项目
深圳市知识创新计划基础研究项目(No.JCYJ20150403101146287)资助 Supported by the Shenzhen Knowledge Innovation Project Basic Research Project (No.JCYJ20150403101146287) （No.JCYJ20150403101146287）
