中国人兽共患病学报2017，Vol.33Issue(12)：1125-1130,6.DOI:10.3969/j.issn.1002-2694.2017.12.014
云南西部地区家畜动物感染巴贝虫的分子流行病学调查
Infection of Babesia in domestic animals in Western Yunnan Province, China
摘要
Abstract
The objective of this study was to investigate Babesia infection among domestic animals in Western Yunnan Province and provide scientific evidence for developing control measures.A total of 1 073 domestic animals blood samples (274cattle,395 sheep,354 dogs,33 horses and 17 donkeys) were collected in 12 counties in Western Yunnan Province.Genomic DNA was extracted and a near full-length 18S rRNA gene sequence of Babesia was amplified by using nested PCR.Babesia species was identified by Blast program and phylogenetic tree.It was indicated that 50 samples were infected with Babesia,belonging to 5 species and with the infection rate of 4.66％.Among 274 cattle blood samples,11 were infected with Babesia (4.01％).Four of them were Babesia bovis and seven of them were Babesia bigemina.Among 395 sheep blood samples,38 were infected with Babesia (9.62 ％),37 of them were Babesia odocoilei-like parasites and 1 of them was Babesia capreoli-like parasites.Horses and donkeys were negative.In conclusion,domestic animals in Western Yunnan Province are infected with many kinds of Babesia,which threaten stock raising development and human health.It is necessary to strengthen prevention of babesiosis and investigate infection rate of babesiosis in human.关键词
云南省/家畜动物/巴贝虫/调查Key words
Yunnan Province/domestic animals/Babesia/investigation分类
医药卫生引用本文复制引用
黄涛华,高子厚,蒋宝贵,赵文红,苏超,贾娜,张青,江佳富,杜春红..云南西部地区家畜动物感染巴贝虫的分子流行病学调查[J].中国人兽共患病学报,2017,33(12):1125-1130,6.基金项目
国家自然科学基金项目(No.81360413,No.81673235,No.81760607)和云南省技术创新人才培养项目(No.2014HB093)联合资助 Supported by grants from the National Natural Science Foundation of China (Nos.81360413,81673235,81760607),and the Yunnan Provincial Training of Youth Leader in Academic and Technical Reserve Talents (No.2014HB093) （No.81360413,No.81673235,No.81760607）
