中国兽医科学2018，Vol.48Issue(1)：1-6,6.DOI:10.16656/j.issn.1673-4696.2018.0001
A型动物流感病毒RT-RPA检测方法的建立及初步应用
Establishment and preliminary applications of the RT-RPA method for detection of type A influenza virus
摘要
Abstract
RPA(recombinase polymerase amplification) assay for detection of type A influenza virus was developed based on M gene nucleotide sequence of type A influenza virus.We verified the designed primers and probes through the specific selection of common pathogens of animal diseases.Then multiple pairs of primers were designed to select optimal reaction system and reaction conditions,and to compare with real-time RT-PCR method in sensitivity.The results showed that the RT-RPA method had strong specificity.It was rapidly tested about 20 minutes for detecting influenza A virus.The detection limit of RT-RPA for virus genome DNA was about 0.96 × 105 μg/mL,slightly lower than the detection limit of real-time RT-PCR.Thus the RT-RPA method detection of type A influenza virus is specific,sensitive,simple,rapid,and more suitable for use in field conditions and primary level laboratories.关键词
A型动物流感病毒/重组酶聚合酶扩增技术(RPA)/等温扩增Key words
type A animal influenza virus/recombinant polymerase amplification (RPA)/isothermal amplification分类
农业科技引用本文复制引用
王潇,曹琛福,林彦星,黄超华,贾伟新,花群义..A型动物流感病毒RT-RPA检测方法的建立及初步应用[J].中国兽医科学,2018,48(1):1-6,6.基金项目
国家十三五重点研发项目(2016YFD0500708) （2016YFD0500708）
