中国兽医科学2018，Vol.48Issue(1)：13-18,6.DOI:10.16656/j.issn.1673-4696.2018.0003
猪传染性胃肠炎病毒N蛋白单克隆抗体的制备及鉴定
Production and detection of monoclonal antibodies against recombinant nucleoprotein of TGEV
摘要
Abstract
BALB/c mice were immunized with purified recombinant N protein(rNp) of porcine transmissible gastroenteritis virus(TGEV).Cellfusion and screening were carried out according to standard methods.Finally,two hybridoma cell lines against TGEVNPwere obtained and named as 2G7 and 2C2,respectively.The isotypes of the two monoclonal antibodies were found to be IgG1.Ascites titer of two monoclonal antibodies was 1∶108.The result of indirect ELISA showed that the antibody titers of supernatant of the 20th passage of cells were 1∶12 800,indicating stable and strong antibody secreting capacity of the two hybridoma.Moreover,the result of immunofluorescent analysis showed high specificity of the two monoclonal antibodies against TGEV.These results demonstrated that the two monoclonal antibodies prepared in our study had high sensitivity,and might provide valuable tools for accurate and rapid diagnosis of TGEV.关键词
传染性胃肠炎病毒/重组N蛋白/单克隆抗体Key words
transmissible gastroenteritis virus(TGEV)/recombinant N protein(rNp)/McAb分类
农业科技引用本文复制引用
王赛,苏菲,袁秀芳,徐丽华,李军星,余兴龙..猪传染性胃肠炎病毒N蛋白单克隆抗体的制备及鉴定[J].中国兽医科学,2018,48(1):13-18,6.基金项目
公益技术研究农业项目(2016C32072) （2016C32072）
杭州市农业科研主动设计项目(20162012A07) （20162012A07）
