中国兽医科学2018，Vol.48Issue(1)：27-33,7.DOI:10.16656/j.issn.1673-4696.2018.0005
鼠源VCAM-1基因TaqMan实时荧光定量PCR检测方法的建立
Development and evaluation of a real-time TaqMan reverse transcription-PCR assay for quantification of murine VCAM-1 gene
摘要
Abstract
Objective to develop a real-time TaqMan reverse transcription-PCR(RT-PCR) assay for effective quantification of murine VCAM-1 gene.Based on the murine VCAM-1 gene sequence published in GenBank,a pair of PCR primers and a probe were designed.Then a real-time TaqMan RT-PCR assay for accurate quantification of murine VCAM-1 gene was developed and optimized.In result,the real-time TaqMan RT-PCR assay had a good linear relationship.The standard curve correlation coefficient r2 was 0.998.It also had a high sensitivity and reproducibility as well as specificity.It can be used to the qualitative identification of murine cells'VCAM-l.In conclusion,a rapid and accurate method for quantification of murine cells'VCAM-1 gene by real-time TaqMan reverse transcription-PCR was successfully developed.关键词
VCAM-1基因/TaqMan探针/实时荧光定量PCR/检测Key words
VCAM-1 gene/TaqMan probe/real-time quantitative PCR/quantification分类
农业科技引用本文复制引用
王兴陇,包晓婧,张海霞,李向茸,冯若飞..鼠源VCAM-1基因TaqMan实时荧光定量PCR检测方法的建立[J].中国兽医科学,2018,48(1):27-33,7.基金项目
国家自然科学基金项目(31460665) （31460665）
中央高校基本科研业务费专项资金项目(3192015005) （3192015005）
中央专项研究生科研创新项目(Yxm2017119) （Yxm2017119）
