中国兽医科学2018，Vol.48Issue(1)：39-47,9.DOI:10.16656/j.issn.1673-4696.2018.0007
甘肃某奶牛场乳腺炎流行病学调查及无乳链球菌血清型分布研究
Epidemiological investigation of mastitis and distribution of Streptococcus agalactiae in a dairy farm in Gansu
摘要
Abstract
An epidemiological investigation of mastitis was carried out in a dairy farm,located in Gansu,to provide the theoretic base for effectively controlling and preventing bovine mastitis.The incidence of clinical and sub-clinical mastitis in the dairy farm was investigated,and 125 milk samples from cows with clinical mastitis were collected and the strains in samples were cultured,separated and identified.Then,the main pathogens were used for drug sensitive test,and the serotype of Streptococcus agalactiae was also identified in this study.The results showed that the incidence rate of clinical mastitis was 9.68％,and 18％were sub-clinical mastitis.128 strains were isolated from milk samples with a high isolation rate (93.6％),including E.coli (n=41,32.03％),S.agalactiae (n=25,19.53％),Enterobacter(n=14,10.94％),and Streptococcus dysgalactiae(n=2,1.56％).Serotype test indicated that 18 strains were I a-type(72.0％),and 7 strains were Ⅱ-type(28.0％).Antibiotic resistance rate revealed that the major pathogens of mastitis were resistant to Clindamycin,Vancomycin and Rifampicin,and the resistance rate was 50％to 100％.However,they were very sensitive to Gentamicin and Ciprofloxacin.This study is significant for the control of bovine mastitis.关键词
奶牛乳腺炎/发病率/病原菌/血清型/耐药性Key words
bovine mastitis/incidence rate/pathogens/serotype/drug resistance分类
农业科技引用本文复制引用
张亚茹,李宏胜,杨峰,李新圃,罗金印,张哲,刘龙海,王丹,朱新荣,杨孝朴..甘肃某奶牛场乳腺炎流行病学调查及无乳链球菌血清型分布研究[J].中国兽医科学,2018,48(1):39-47,9.基金项目
国家重点研发项目(2017YFD0502200) （2017YFD0502200）
甘肃省重点研发计划-国际科技合作专项(2017GS11063) （2017GS11063）
中国农业科学院基本科研业务费专项院级统筹(Y2016PT43) （Y2016PT43）
中国农业科学院奶牛疾病研究创新工程项目(CAAS-ASTIP-2014-LIHPS-03) （CAAS-ASTIP-2014-LIHPS-03）
