中国现代医学杂志2018，Vol.28Issue(4)：11-16,6.DOI:10.3969/j.issn.1005-8982.2018.04.003
后膜骨架蛋白1a在小鼠脑缺血再灌注损伤中的作用
Study on effect of Homer 1a on cerebral ischemia-reperfusion injury in mice
杨小利 1殷亚楠 2胡文淼 1张理斐 1童丽 1孙志平 1程坤 1王亚君1
作者信息
- 1. 河南省郑州市第三人民医院 儿科,河南 郑州 450000
- 2. 河南省郑州市第二人民医院 神经内科,河南 郑州 450000
- 折叠
摘要
Abstract
Objective To investigate the effect of Homer 1a on cerebral ischemia-reperfusion injury in mice. Methods Homer 1a knockout mice were used as the research objects to construct the model of cerebral ischemia-reperfusion in mice. The degree of cerebral ischemia-reperfusion injury in mice was evaluated based on the behavioral score. TTC staining was used to observe and calculate the volume of cerebral infarction. Apoptosis of the brain tissues of the mice was detected by Tunel. The proliferation of astrocytes was detected by GFAP method. The levels of IL-1β, TNF-α and IL-6 in the mouse brain were detected by Western blot. Results The behavioral score of the experimental group was significantly higher than that of the cerebral ischemia group, indicating that cerebral ischemia-reperfusion injury of the Homer 1a gene knockout mice were more severe than that of the cerebral ischemia group. The cerebral infarction volume in the experimental group was significantly larger than that in the cerebral ischemia group (P < 0.05). The apoptosis rate of brain cells in the experimental group was significantly higher than that in the cerebral ischemia group (P < 0.05). The number of brain astrocytes in the experimental group and the cerebral ischemia group was larger than that in the normal group (P < 0.05), while the number of brain astrocytes in the experimental group was smaller than that in the cerebral ischemia group. The expression levels of IL-6, TNF-α and IL-1β in the brain tissue of the experimental group were higher than those in the cerebral ischemia group (P < 0.05). Conclusions Homer 1a knockout can aggravate the injury after cerebral ischemia and reperfusion in mice. Homer 1a can attenuate cerebral ischemia-reperfusion injury.关键词
脑缺血/Homer1a/星形胶质细胞/基因敲除Key words
cerebral ischemia/Homer 1a/astrocyte/gene knockout分类
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杨小利,殷亚楠,胡文淼,张理斐,童丽,孙志平,程坤,王亚君..后膜骨架蛋白1a在小鼠脑缺血再灌注损伤中的作用[J].中国现代医学杂志,2018,28(4):11-16,6.
