中国现代医学杂志2018，Vol.28Issue(4)：17-21,5.DOI:10.3969/j.issn.1005-8982.2018.04.004
微针对5-氨基酮戊酸经皮渗透的作用研究
Effect of microneedle on transdermal permeation of 5-aminolevulinic acid
摘要
Abstract
Objective To determine the content of protoporphyrin IX (PpIX) in chicken comb tissues after using microneedles and topical application of 5-aminolevulinic acid (5-ALA), so as to observe whether microneedles can help 5-ALA permeate through epidermis of the comb. Methods Using chicken comb as the animal modle of port wine stain, the chickens were divided into ALA group and microneedle combined with ALA group. In the ALA group, 5-ALA was applied only; in the microneedle combined with ALA group, the chicken comb was applied with 5-ALA after grinding with microneedles; both groups used untreated side as self-control. The localization of PpIX was observed under inverted fluorescence phase contrast microscope, and the fluorescence intensity of PpIX was calculated by fluorospectrophotometer. Results In the comb, PpIX content increased gradually after applying ALA and reached the peak level at 4 hours in the two groups, and obviously decreased after 6 hours, but the PpIX level in the microneedle combined with ALA group was significantly higher than that in the ALA group (P < 0.05). Inverted fluorescence phase contrast microscopy showed that PpIX distributed evenly in the vascular tissue of epidermis and dermis, and the fluorescent intensity in the microneedle combined with ALA group was stronger than that in the ALA group. Conclusions Microneedles can help 5-ALA better permeate through epidermis. This experiment also provides reference base for optimizing 5-ALA usage in clinic.关键词
5-氨基酮戊酸/鲜红斑痣/原卟啉IX/微针/鸡冠Key words
5-aminolevulinic acid/port wine stain/protoporphyrin IX/microneedle/chicken comb分类
医药卫生引用本文复制引用
梁齐飞,云健健,杨凤元,黄熙..微针对5-氨基酮戊酸经皮渗透的作用研究[J].中国现代医学杂志,2018,28(4):17-21,5.基金项目
广西自然科学基金面上项目(No:桂科计字[2016]380号) （No:桂科计字[2016]380号）
