中国现代医学杂志2018，Vol.28Issue(4)：26-29,4.DOI:10.3969/j.issn.1005-8982.2018.04.006
精液凝固蛋白Ⅰ活性片段的精子抑制活性分析
Analysis of sperm inhibitory activity of semenogelin I active fragment
摘要
Abstract
Objectlve To prepare the recombinant semenogelin (Sg) and its different small molecule derived peptides and study the effect of Sg on sperm motility. Methods Molecular cloning and PCR amplification were used to prepare the recombinant Sg, then the recombinant protein was used to test the activity of male sperm with normal reproductive function. Results At the concentration of 200 pg/sperm, the N-terminal fragment of the recombinant Sg had the effect of inhibiting sperm motility (P < 0.05), and the C-terminal fragment also inhibited sperm motility (P < 0.05). Conclusions There are amino acid fragments in the Sg molecule that can inhibit sperm motility, Sg24-163 has the highest sperm inhibitory activity.关键词
精液凝固蛋白/精子活动/抑制活性/蛋白重组Key words
semenogelin/sperm motility/inhibitory activity/recombinant technique分类
医药卫生引用本文复制引用
韩飞,孙凯,赵晖..精液凝固蛋白Ⅰ活性片段的精子抑制活性分析[J].中国现代医学杂志,2018,28(4):26-29,4.基金项目
云南省科技厅-昆明医科大学应用基础研究联合专项基金项目(No:2014FB031) （No:2014FB031）
