中国现代医学杂志2018，Vol.28Issue(4)：39-44,6.DOI:10.3969/j.issn.1005-8982.2018.04.009
MicroRNA-21、microRNA-137和microRNA-498在肺腺癌患者血清中表达情况及其临床意义
miR-498, miR-21 and miR-137 as potential serum markers in diagnosis of lung adenocarcinoma
摘要
Abstract
Objective To study the expressions of lung adenocarcinoma-related miRNAs in the serum of normal population and lung adenocarcinoma patients, and analyze their clinical value in the diagnosis of lung adenocarcinoma. Methods Serums were collected from 40 normal people and 59 lung adenocarcinoma patients. qRT-PCR was used to test the expressions of miR-498, miR-21, miR-137, miR-206, miR-138, miR-32 and miR-125b. The value of single serum miRNA in diagnosis of lung adenocarcinoma was analyzed and the diagnostic value of combined detection of multiple miRNAs were further analyzed using statistical methods. Results Compared with the normal controls, the expressions of miR-498, miR-21 and miR-137 were increased in the serum of the lung adenocarcinoma patients (P < 0.05). Meanwhile, the expressions of miR-206, miR-138, miR-32 and miR-125b hadno statistical differences between the two groups. AUC analysis showed that, the AUC value of miR-498 was 0.73 (95％ CI: 0.63-0.81, P < 0.05); the AUC value of miR-21 was 0.83 (95％ CI: 0.74-0.90, P < 0.05); the AUC value of miR-137 was 0.74 (95％ CI: 0.64-0.82, P < 0.05). The AUC value of miR-498, miR-21 combined with miR-137 in the diagnosis of lung adenocarcinoma was 0.93 (95％ CI: 0.85-0.97, P < 0.05), which was better than any of three miRNAs (P < 0.05). Conclusions The expressions of serum miR-498, miR-21 and miR-137 in the lung adenocarcinoma patients are elevated, and combination of miR-498, miR-21 and miR-137 can be used as an potential marker for the diagnosis of lung adenocarcinoma.关键词
肺腺癌/微小RNA/诊断Key words
lung adenocarcinoma/miRNA/diagnosis分类
医药卫生引用本文复制引用
唐曦,胡娅,徐炎华,汪春林,邱萍,王向辉..MicroRNA-21、microRNA-137和microRNA-498在肺腺癌患者血清中表达情况及其临床意义[J].中国现代医学杂志,2018,28(4):39-44,6.基金项目
湖北省卫生厅项目(No:2012Z-Y31) （No:2012Z-Y31）
湖北省教育厅项目(No:B2013280) （No:B2013280）
