中国现代医学杂志2018，Vol.28Issue(4)：57-61,5.DOI:10.3969/j.issn.1005-8982.2018.04.012
MicroRNA-204在非小细胞肺癌患者组织中的表达及对癌细胞增殖和凋亡的影响
miRNA-204 expression in non-small cell lung cancer tissue and its effect on cancer cell proliferation and apoptosis
许有忠 1田作春 1李才1
作者信息
- 1. 海南省第三人民医院 心胸外科,海南 三亚 572000
- 折叠
摘要
Abstract
Objective To observe the expression of miRNA-204 in the lung tissue of non-small cell lung cancer (NSCLC) patients and its effect on lung cancer cell proliferation and apoptosis. Methods qRT-PCR was used to detect the expression of miRNA-204 in the lung cancer and peri-cancerous tissues. After A-549 cells were interfered with miRNA-204 mimics, the cell proliferation was observed using CCK-8, cell apoptosis was observed by flow cytometry, and the mRNA and protein levels of Bcl-2 were detected by qRT-PCR and Western blot respectively. Results The expression of miRNA-204 in the NSCLC tissues was significantly lower than that in the peri-cancerous tissues (P < 0.05). And the low-expression of miRNA-204 in the NSCLC tissues was associated with tumor size and lymph node metastasis (P < 0.05). After interference of miRNA-204 mimics in the A-549 cells, the cell proliferation was reduced and the cell apoptosis was increased, and the mRNA and protein levels of Bcl-2 were decreased (P < 0.05). Conclusions The expression of miRNA-204 in the NSCLC tissues is decreased and related to the malignant clinicopathological features of lung cancer. miRNA-204 may affect the proliferation and apoptosis of non-small cell lung cancer cells by regulating Bcl-2 expression.关键词
非小细胞肺癌/MicroRNA-204/细胞增殖/细胞凋亡/Bcl-2Key words
non-small cell lung cancer/miRNA-204/cell proliferation/cell apoptosis/Bcl-2分类
医药卫生引用本文复制引用
许有忠,田作春,李才..MicroRNA-204在非小细胞肺癌患者组织中的表达及对癌细胞增殖和凋亡的影响[J].中国现代医学杂志,2018,28(4):57-61,5.
