中国现代医学杂志2018，Vol.28Issue(5)：1-6,6.DOI:10.3969/j.issn.1005-8982.2018.05.001
七氟烷对大鼠近期空间回忆能力、海马APOE、Aβ 表达的影响
Impact of Sevoflurane on short-term spatial memory capacity and expressions of APOE and amyloid beta in hippocampus of rats
摘要
Abstract
Objective To investigate the impact of Sevoflurane on spatial memory capacity and expressions of apolipoprotein E (APOE) and amyloid beta (Aβ) in hippocampus of adult rats. Methods Fifty-four adult rats were randomly divided into sev group, con group and blank group. The rats in the sev group inhaled 3.2％ Sevoflurane and carrier gas (1L/min O2 plus 1L/min air) for 2 h, those in the con group inhaled carrier gas for 2 h and those in the blank group were bred naturally. In all rats Morris Water Maze experiment was done before modeling and sample-taking. Brain tissues were taken on the 1st day (T1), 3rd day (T3) and 7th day (T7) after modeling, then the levels of mean optical density (MOD) of APOE and Aβ in the CA1, CA3 and DG areas of hippocampus were measured by immunohistochemistry (IHC). Results There was no significant difference between the con group and the sev group in the times across the original platform, the times onto original platform quadrant, the retention time in the original platform quadrant or the percentage of the time retaining in the original platform quadrant at each time point (P > 0.05). Compared with the con group, APOE MOD in each hippocampal area of the sev group reduced at T1 and T3 (P < 0.05), Aβ MOD in each hippocampal area of the sev group increased at T7 (P < 0.05). Conclusions 3.2％Sevoflurane does not damage short-term spatial memory ability of adult rats. 3.2％ Sevoflurane inhalation anesthesia inhibits Aβ deposition in hippocampus through down-regulation of APOE expression within 1 week; the 7th day after Sevoflurane anesthesia is a key point for Aβ increase in hippocampus.关键词
七氟烷/海马/载脂蛋白E/β淀粉样蛋白Key words
Sevoflurane/hippocampus/apolipoprotein E/amyloid beta分类
医药卫生引用本文复制引用
徐珊,朱昭琼,王义,唐春春,张超..七氟烷对大鼠近期空间回忆能力、海马APOE、Aβ 表达的影响[J].中国现代医学杂志,2018,28(5):1-6,6.基金项目
国家自然科学基金(No :81360180) （No :81360180）
