中成药2018，Vol.40Issue(1)：14-20,7.DOI:10.3969/j.issn.1001-1528.2018.01.003
大川芎方效应组分对偏头痛大鼠降钙素基因相关肽及其受体表达的影响
Effects of active components extracted from Dachuanxiong Decoction on the expressions of CGRP and its receptors in rats with migraine
摘要
Abstract
AIM To study the effects of active components extracted from Dachuanxiong Decoction (DCXD,Chuanxiong Rhizoma,Gastrodiae Rhizoma) on the expressions of calcitonin gene-related peptide (CGRP) and its receptors in hypothalamus and periaqueductal gray (PAG) of rats with migraine.METHODS Seventy-two rats were evenly assigned to six groups at random,normal group,model group,positive drug group,groups of highdose,middle-dose and low-dose DCXD.Each group was then divided into Subgroup A and Subgroup B.The migraine rat model was established by subcutaneous nitroglycerin injection.The expressions of CGRP in Subgroup B's rat hypothalamus and PAG were observed by immunohistochemical staining,while the levels of CGRP in rat plasma,hypothalamus and PAG in Subgroup A were detected by ELISA.All the gene expressions of CGRP and its receptors (CRLR,RCP,RAMP1) in Subgroup A's rat hypothalamus and PAG were assessed by real-time PCR.RESULTS There was a more significant CGRP level increase in plasma,hypothalamus and PAG in the model group than that in the normal group (P ＜ 0.01).The modelled rats found their up-regulated expressions of CGRP and RCP in hypothalamus (P ＜ 0.05),up-regulated expressions of CGRP and CRLR in PAG,and down-regulated expression of RCP (P ＜ 0.05).All low-dose,middle-dose,high-dose active components worked in decreasing the CGRP levels in plasma,hypothalamus and PAG.The middle-dose led to down-regulation of CGRP,RCP expressions in hypothalamus (P ＜0.05,P ＜0.01),down-regulation of CGRP,CRLR expressions in PAG (P ＜0.05),and up-regulation of RCP expression in PAG (P ＜ 0.05).CONCLUSION The mechanism of active components extracted from DCXD in migraine management may associate with its capability in down-regulating CGRP expressions in hypothalamus and PAG,reducing CGRP synthesis and inhibiting neurogenic inflammation.关键词
大川芎方效应组分/CGRP/下丘脑/PAGKey words
active components extracted from Dachuanxiong Decoction/CGRP/hypothalamus/PAG分类
医药卫生引用本文复制引用
王清清,沈岚,张俊,林晓,冯怡..大川芎方效应组分对偏头痛大鼠降钙素基因相关肽及其受体表达的影响[J].中成药,2018,40(1):14-20,7.基金项目
上海市卫生和计划生育委员会项目资助(ZY3-CCCX-3-5001) （ZY3-CCCX-3-5001）
上海市科学技术委员会平台资助(15DZ2292000) （15DZ2292000）
浦东新区科技发展基金资助(PKF-2003-003) （PKF-2003-003）
