中成药2018，Vol.40Issue(1)：20-26,7.DOI:10.3969/j.issn.1001-1528.2018.01.004
消定膏对骨折愈合过程中COX-2/PGE2/cAMP信号通路表达的影响
Effects of Xiaoding Ointment on COX-2/PGE2/cAMP signal pathway expression in fracture healing
摘要
Abstract
AIM To study the effects and action mechnism of Xiaoding Ointment (Pyrolusite,Catechu,Rhei Radix et Rhizoma,etc.) on rabbits' fracture healing in terms of cyclooxygenase-2 (COX-2),prostaglandin E2 (PGE2),cyclic adenosine monophosphate (cAMP) expressions at different time points.METHODS One hundred and twenty-eight New Zealand white rabbits were randomly divided into blank group,model group,Qingpeng Ointment (Oxytropis Falcatae Herba,Rhei lhasaense Radix et Rhizoma,Terminalia chebula Fructus,etc.) group and Xiaoding Ointment group.All the other groups,except the blank group,were made with ulna 3 mm bone defect,after which external fixation was applied to both the blank group and the model group.On the 3rd,7th,14th and 28th days after the medications,eight rabbits randomly selected from various groups had their callus morphopathology changes observed under optical microscope,their callus tissue COX-2 mRNA expression levels were detected by realtime fluorescence quantitative polymerase chain reaction (qRT-PCR),and their callus tissue PGE2 and cAMP protein expressions were determined by immunohistochemistry.RESULTS Compared with the model group and the Qingpeng Ointment group,the Xiaoding Ointment group exhibited significantly better formation of callus and collagen fibers.The mRNA expression of COX-2,and the protein expressions of PGE2 and cAMP in the Xiaoding Ointment group were remarkably enhanced as revealed on the 7th and 14th days' postoperative check (P ＜ 0.05),and the peak values arrived around the 14th day after the operation.CONCLUSION Xiaoding Ointment's obvious effect on promoting fracture healing may associate with its impact on COX-2/PGE2/cAMP signaling pathway.关键词
消定膏/骨缺损/环氧化酶-2(COX-2)/前列腺素E2(PGE2)/环磷酸腺苷(cAMP)Key words
Xiaoding Ointment/bone defect/cyclooxygenase-2 (COX-2)/prostaglandin E2 (PGE2)/cyclic adenosine monophosphate (cAMP)分类
医药卫生引用本文复制引用
董万涛,宋敏,陈秉雄,巩彦龙,刘涛,张洁,刘保健,冯宜蒀..消定膏对骨折愈合过程中COX-2/PGE2/cAMP信号通路表达的影响[J].中成药,2018,40(1):20-26,7.基金项目
甘肃省高校科研项目(2016B-056) （2016B-056）
吴阶平医学基金会临床科研专项基金资助项目(320.6750.11061) （320.6750.11061）
甘肃省青年自然科学基金计划(1208RJYA066) （1208RJYA066）
