中成药2018，Vol.40Issue(1)：101-105,5.DOI:10.3969/j.issn.1001-1528.2018.01.019
UPLC-QQQ-MS法同时测定片仔癀中13种成分
Simultaneous determination of thirteen constituents in Pientzehuang by UPLC-QQQ-MS
摘要
Abstract
AIM To establish a ultra-high performance liquid chromatography coupled with triple quadruple mass spectrometry (UPLC-QQQ-MS) method for the simultaneous content determination of notoginsenoside R1,ginsenoside Re,ginsenoside Rg1,ginsenoside Rb1,ginsenoside Rd,taurine,taurocholic acid,cholic acid,glycocholic acid,glycodeoxycholicacid,chenodeoxycholic acid,deoxycholic acid and muscone in Pientzehuang (Bovis Calculus,Moschus,Notoginseng Radix et Rhizoma,etc.).METHODS The analysis of methanol extract of this drug was performed on a 40 ℃ thermostatic Waters CORTECS UPLC C18column (100 mm × 2.1 mm,1.6 μm),with the mobile phase comprising of acetonitrile-water (containing 0.1％ formic acid) flowing at 0.25 mL/min in a gradient elution manner.RESULTS Thirteen constituents showed good linear relationships within their own ranges (r ＞0.998 5),whose average recoveries were 91.1％-105.3％ with the RSDs of 2.4％-4.6％.CONCLUSION This accurate,simple,sensitive and reproducible method can be used for the quality control of Pientzehuang.关键词
片仔癀/化学成分/UPLC-QQQ-MSKey words
Pientzehuang/chemical constituents/UPLC-QQQ-MS分类
医药卫生引用本文复制引用
黄秋妹,邰艳妮,朱艳琳,沈少君,郭惠婷,黄鸣清..UPLC-QQQ-MS法同时测定片仔癀中13种成分[J].中成药,2018,40(1):101-105,5.基金项目
福建省教育厅高校新世纪优秀人才支持计划(闽教科[2015] 54) （闽教科[2015] 54）
福建省大学生创新创业训练项目(201610393010) （201610393010）
