中成药2018，Vol.40Issue(1)：105-109,5.DOI:10.3969/j.issn.1001-1528.2018.01.020
LC-MS/MS法同时测定护心口服液中11种成分
Simultaneous determination of eleven constituents in Huxin Oral Liquid by LC-MS/MS
摘要
Abstract
AIM To establish a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the simultaneous content determination of matrine,oxymatrine,salvia acid B,tanshinol,protocatechualdehyde,liquiritin,astragaloside,cinnamaldehyde,tanshinone Ⅱ A,ophiopogonin D and ruscogenin in Huxin Oral Liquid (Glycyrrhizae Radix et Rhizoma,Astragali Radix,Ophiopogonis Radix,etc.).METHODS The analysis of methanol extract of this drug was performed on a 25 ℃ thermostatic Ultimate XB-C18 column (4.6 mm × 100 mm,3 μm),with the mobile phase comprising of water-methanol (containing 0.05％ formic acid) flowing at 0.4 mL/min in a gradient elution manner.RESULTS Eleven constituents showed good linear relationships with-in their own ranges (r ＞0.999 0),whose average recoveries were 96.66％-103.2％ with the RSDs of 1.4％-3.4％.CONCLUSION This sensitive,reliable and accurate method can be used for the quality control of Huxin Oral Liquid.关键词
护心口服液/化学成分/LC-MS/MSKey words
Huxin Oral Liquid/chemical constituents/LC-MS/MS分类
医药卫生引用本文复制引用
梁琨,孙辉,安叡,王新宏,尤丽莎,蔡征宇..LC-MS/MS法同时测定护心口服液中11种成分[J].中成药,2018,40(1):105-109,5.基金项目
上海市黄浦区优秀学科带头人培养项目([2013] HWR/D-13) （[2013] HWR/D-13）
