中成药2018，Vol.40Issue(1)：114-117,4.DOI:10.3969/j.issn.1001-1528.2018.01.022
HPLC法同时测定四磨汤口服液中5种成分
Simultaneous determination of five constituents in Simotang Oral Liquid by HPLC
摘要
Abstract
AIM To establish an HPLC method for the simultaneous content determination of five constituents in Simotang Oral Liquid (Aucklandiae Radix,Aurantii Fructus,Arecae Semen,etc.).METHODS The analysis of 50％ methanol extract of this drug was performed on a 35 ℃ thermostatic Agilent Zorbax C18 column (4.6 mm × 250 mm,5 μm),with the mobile phase comprising of acetonitrile-0.1％ formic acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 283 nm.RESULTS Synephrine,norisoboldine,naringin,hesperidin and neohesperidin showed good linear relationships within the ranges of 5.8-185.6 μg/mL (r =0.999 9),0.829-26.52 μg/mL (r =1.000 0),9.775-312.8 μg/mL (r =1.000 0),0.594-19 μg/mL (r =0.999 5) and 5.2-166.4 μg/mL (r =1.000 0),whose average recoveries were 98.93％,98.95％,102.57％,99.67％ and 103.43％ with the RSDs of 1.85％,1.27％,0.52％,0.89％ and 0.43％,respectively.CONCLUSION This simple,accurate and reproducible method can be used for the rapid quality control of Simotang Oral Liquid.关键词
四磨汤口服液/辛弗林/去甲异波尔定/柚皮苷/橙皮苷/新橙皮苷/HPLCKey words
Simotang Oral Liquid/synephrine/norisoboldine/naringin/hesperidin/neohesperidin/HPLC分类
医药卫生引用本文复制引用
张卫东,戴国梁,白永涛,朱立静,井汶,居文政..HPLC法同时测定四磨汤口服液中5种成分[J].中成药,2018,40(1):114-117,4.基金项目
国家自然科学基金资助项目(81573685) （81573685）
