中成药2018，Vol.40Issue(1)：117-120,4.DOI:10.3969/j.issn.1001-1528.2018.01.023
HPLC法同时测定白芍配方颗粒中5种成分
Simultaneous determination of five constituents in Baishao Formula Granules by HPLC
摘要
Abstract
AIM To establish an HPLC method for the simultaneous content determination of five constituents in Baishao Formula Granules (Paeoniae Radix Alba).METHODS The analysis of aqueous extract of this drug was performed on a 30 ℃ thermostatic Phenomenex C18 column (4.6 mm × 250 mm,5 μm),with the mobile phase comprising of acetonitrile-0.05％ phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 230 nm.RESULTS Paeoniflorin,albiflorin,paeoniflorin sulfonate,gallic acid and benzoic acid showed good linear relationships within the ranges of 0.020-0.639 mg/mL (r =0.999 8),0.005-0.172 mg/mL (r =0.999 9),0.020-0.652 mg/mL (r =1.000 0),0.003-0.097 mg/mL (r =0.999 8),0.002-0.058 mg/mL (r =0.999 7),whose average recoveries were 99.1％,98.3％,98.6％,98.1％ and 99.5％ with the RSDs of 1.86％,1.37％,1.69％,1.46％ and 2.26％,respectively.The contents of various constituents in twenty-seven batches of samples demonstrated obvious differences.CONCLUSION We should pay attention to Paeoniae Radix Alba in Baishao Formula Granules due to its unstable quality.关键词
白芍配方颗粒/芍药苷/芍药内酯苷/芍药苷亚硫酸酯/没食子酸/苯甲酸/HPLCKey words
Baishao Formula Granules/paeoniflorin/albiflorin/paeoniflorin sulfonate/gallic acid/benzoic acid/HPLC分类
医药卫生引用本文复制引用
钱珺,谢凡,石燕红,李文艳,王瑞..HPLC法同时测定白芍配方颗粒中5种成分[J].中成药,2018,40(1):117-120,4.基金项目
国家自然科学基金(81703698) （81703698）
国家药品标准提高暨2015版药典科研任务(2012) （2012）
