中成药2018，Vol.40Issue(1)：121-125,5.DOI:10.3969/j.issn.1001-1528.2018.01.024
大花吴茱萸果实化学成分及其生物活性
Chemical constituents from the fruits of Evodia rutaecarpa and their biological activities
摘要
Abstract
AIM To study the chemical constituents from the fruits of Evodia rutaecarpa (Juss.) Benth.and their biological activities.METHODS The ethyl acetate fraction of 80％ ethanol extract from E.rutaecarpa was isolated and purified by silica column and recrystallization,then the structures of obtained compounds were identified by physicochemical properties and spectral data.The antitumor and antifungal activities were determined by MTT and mycelium growth method,respectively.RESULTS Ten compounds were isolated and identified as rutaecarpine (1),evodiamine (2),1-methyl-2-undecyl-4 (1H)-quinolone (3),1-methyl-2-tridecyl-4 (1H)-quinolone (4),wuchuyuamide Ⅰ (5),wuchuyuamide Ⅲ (6),wuchuyuamide Ⅳ (7),β-sitosterol (8),β-daucosterol (9),dehydroevodiamine (10).Compounds 1,2,10 had certain inhibitory effects on MDA-MB-231,LoVo,A2780 and HeLa with the IC50values of 0.65-29.45 μmol/L.Compound 1 had a certain inhibitory effect on Botrytis cinerea,Glomerella cingulata and Rhizoctonia solani with the inhibitory rates of 18.12％-49.57％.CONCLUSION Compounds 3-7 are isolated from this plant for the first time,compounds 1,2,10 have strong biological activities.关键词
大花吴茱萸/果实/化学成分/分离鉴定/抗肿瘤活性/抗真菌活性Key words
Evodia rutaecarpa (Juss.) Benth./fruits/chemical constituents/isolation and identification/antitumor activity/antifungal activity分类
医药卫生引用本文复制引用
芮伟,冯煦,刘飞,印敏,王鸣,汤辰炜,罗金岳,王奇志..大花吴茱萸果实化学成分及其生物活性[J].中成药,2018,40(1):121-125,5.基金项目
国家自然科学基金面上项目(31570359,31470425) （31570359,31470425）
江苏省科技计划项目(BM2015019) （BM2015019）
