中国现代中药2017，Vol.19Issue(12)：1683-1687,5.DOI:10.13313/j.issn.1673-4890.2017.12.005
双边栝楼成熟果实不同部位游离氨基酸及核苷类资源性化学成分分析与评价
Analysis and Evaluation of Amino Acids and Nucleosides in Different Parts of Ripe Fruit of Trichosanthes rosthornii Harms
摘要
Abstract
Objective:To provide the theoretical basis for the comprehensive utilization of Trichosanthes rosthornii Harms,nucleosides and amino acids in different parts of T.rosthornii (peel,pulp and seed) were analyzed and evaluated.Methods:Ultra performance liquid chromatography-mass spectrometry (UPLC/MS-MS) technique was used to determine the contents of nucleosides and dissociative amino acids of different parts in T.rosthornii planted in Anhui.Results:In the samples of T.rosthornii,19 kinds of amino acids were detected.The total amount of amino acids is sorted as fruit peel (3.23％) ＞ fruit pulp (2.22％) ＞ seed (0.591％).The main amino acids in fruit pulp are arginine,citrulline,serine,proline.The total amount of the four accounts for about 62％ of the total amino acid content.Citrulline,arginine,lysine and glutamine were at a high levels in fruit peel.The total amount of the four accounted for about 81％ of the total amino acid content.The content of arginine in the seeds was relatively higher,and the content of other amino acids was less than 0.1％.The total amount of thymidine and cytidine was sorted as fruit peel (116.9 μg ·g-1) ＞ fruit pulp (38.09 μg ·g-1) ＞ seed (23.38 μg ·g-1).Conclusion:The different parts of T.rosthornii (peel,pulp and seed) contain a number of dissociative amino acids and nucleosides,with potential medicinal and nutritional value.Especially,the pulp which is the waste in initial processing in origins is worth in-depth development and utilization.关键词
双边栝楼/果实/化学成分分析/资源化利用评价Key words
Trichosanthes rosthornii Harms/fruit/chemical composition analysis/resource utilization evaluation引用本文复制引用
张黄琴,刘培,朱振华,董玲,李卫文,钱大玮,段金廒..双边栝楼成熟果实不同部位游离氨基酸及核苷类资源性化学成分分析与评价[J].中国现代中药,2017,19(12):1683-1687,5.基金项目
江苏高校优势学科建设工程项目(PAPD) （PAPD）
江苏省“333高层次人才培养工程”项目 （）
公益性行业科研专项(201407002) （201407002）
