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独活种子生活力测定的四唑染色法及其与发芽率的相关性研究

钟淑梅 郭晓亮 郭杰 游景茂

中国现代中药2017,Vol.19Issue(12):1732-1734,3.
中国现代中药2017,Vol.19Issue(12):1732-1734,3.DOI:10.13313/j.issn.1673-4890.2017.12.015

独活种子生活力测定的四唑染色法及其与发芽率的相关性研究

Study on Viability Determination using TTC and the Correlation between Viability and Germination Rate of Angelica pubescens Seed

钟淑梅 1郭晓亮 2郭杰 1游景茂1

作者信息

  • 1. 湖北省农业科学院中药材研究所/湖北省农业科技创新中心,湖北恩施445000
  • 2. 九州天润(武汉)中药研究院有限公司,湖北 武汉430000
  • 折叠

摘要

Abstract

Objective:To screen the optimized methods for detection seed viability of Angelica pubescens seed,and determine the relationship between seed viability and germination rate.Method:The effect of dyeing in different concentrations,temperature and time conditions was compared.Use 15 seeds from different sources as a material,the determination of viability and germination rate were tested.Results:The optimum condition of determination seed viability using TIC is soaking seeds in the 1% TTC solution for 50 minutes under the condition of 45 ℃.The regression equation was found out between seed viability (X) and germination rate (Y),which was Y =0.7326 X-0.09 % . Conclusion:The optimum condition of determination seed viability using TIC is soaking seeds in the 1% TTC solution for 50 minutes under the condition of 45 ℃.Furthermore,seed viability was significant related with germination rate of Radix Angelica pubescens seed.

关键词

独活种子/生活力/发芽率/相关性

Key words

Angelica pubescens seed/viability/germination rate/correlation

引用本文复制引用

钟淑梅,郭晓亮,郭杰,游景茂..独活种子生活力测定的四唑染色法及其与发芽率的相关性研究[J].中国现代中药,2017,19(12):1732-1734,3.

基金项目

工业和信息化部2014年中药材生产基地建设扶持项目:道地药材巴东独活规范化生产基地建设 ()

中国现代中药

OACSTPCD

1673-4890

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