中国现代中药2017，Vol.19Issue(12)：1743-1749,1753,8.DOI:10.13313/j.issn.1673-4890.2017.12.018
苯酚-硫酸法联合DNS法测定商陆多糖及响应面法优化超声波辅助提取研究
Study on Determination of Polysaccharides in Radix Phytolaccae by Phenol-Sulfuric Acid Method Combined with DNS Method and Optimization of Ultrasonic Assisted Extraction by Response Surface Methodology
摘要
Abstract
Objective:To find a quick and simple method for determination of polysaccharides in Radix Phytolaccae.Methods:UV spectrophotometry was used to determine the total sugar content by phenol-sulfuric acid method,DNS method was used to determine the content of reducing sugar,and the difference between the two methods was used to measure the content of polysaccharide,and the methods of repeatability,stability and recovery rate were investigated.Based on the single factor study of ultrasonic assisted extraction of polysaccharides from Radix Phytolaccae,the response rate of polysaccharide was taken as the response value,and the response surface experiment design with four factors and three levels was used to optimize the process.Results:The phenol-sulfuric acid combined with DNS method for determination of polysaccharides in Radix Phytolaccae showed a good applicability.The optimized extraction process was as follows:solid-liquid ratio 1∶40(g∶mL),ultrasonic time 30 min,ultrasonic temperature 60 ℃,ultrasonic power 350 W.Under these conditions,the average extraction rate of polysaccharides was 12.38％ ± 0.59％.Conclusion:The method is simple and reliable,and can be used for the determination of polysaccharides in Radix Phytolaccae.关键词
商陆多糖/二硝基水杨酸法/苯酚-浓硫酸法/响应面试验设计/超声波辅助提取Key words
Polysaccharides in Radix Phytolaccae/DNS method/phenol-sulfuric method/response surface methodology/ultrasonic assisted extraction引用本文复制引用
杨俊杰,严进,陆兔林..苯酚-硫酸法联合DNS法测定商陆多糖及响应面法优化超声波辅助提取研究[J].中国现代中药,2017,19(12):1743-1749,1753,8.基金项目
国家中医药行业专项(2015468002-2) （2015468002-2）
信阳市科技攻关项目(150089) （150089）
