中国现代中药2017，Vol.19Issue(12)：1758-1761,1770,5.DOI:10.13313/j.issn.1673-4890.2017.12.021
HPLC波长切换法同时测定金嗓散结胶囊中的(R,S)-告依春、羟基红花黄色素A、丹酚酸B、木蝴蝶苷B和木蝴蝶苷A
Simultaneous Determination of (R, S)-goitrin, Hydroxysafflor Yellow A, Salvianolic acid B, Baicalein-7-O-diglucoside and Baicalin-7-glucoside in Jinsang Sanjie Jiaonang by HPLC Wavelength Switching Method
摘要
Abstract
Objective:To develop a method for simultaneous determination of the content of (R,S)-goitrin,hydroxysafflor yellow A,salvianolic acid B,baicalein-7-0-diglucoside and baicalin-7-glucoside in Jinsang Sanjie Jiaonang.Methods:An HPLC method was adopted for the analysis,which was performed on a Agilent HC-C18 (250 mm ×4.6 mm,5.0 μm)chromatographic column with the mobile phase of methanol-acetonitrile(3∶1)(A)-0.4％ phosphoric acid solution(B) in a gradient elution,the flow rate of 1.0 mL ·min-1,and the samples were preprocessed with ultrasonic extraction in 50％ methanol solution.Results:The linearity ranges of(R,S)-goitrin,hydroxysafflor yellow A,salvianolic acid B,baicalein-7-O-diglucoside and baicalin-7-glucoside were 4.150-83.00 μg· mL-1 (r =0.999 2),5.120-102.4 μg · mL-1 (r =0.999 9),11.07-221.4 μg·mL-1 (r =0.999 4),4.590-91.80 μg·mL-1(r =0.999 7),8.140-162.8 μg·mL-1(r =0.999 5),respectively.The five components were separated perfectly without disturbing peaks from negative samples.The average recoveries and the corresponding RSD were 99.06％ (1.27％),99.54％ (1.15％),98.19％ (0.89％),97.88％ (1.71％),97.22％ (0.99％),respectively.Conclusion:The method can be applied to the quality control of Jinsang Sanjie Jiaonang.关键词
金嗓散结胶囊/(R,S)-告依春/羟基红花黄色素A/丹酚酸B/木蝴蝶苷B/木蝴蝶苷A/波长切换法/中成药多组分测定Key words
Jinsang Sanjie Jiaonang/(R, S)-goitrin/hydroxysafflor yellow A/salvianolic acid B/baicalein-7-O-diglucoside/baicalin-7-glucoside/wavelength switching method/multicomponent determination of Chinese patent drug引用本文复制引用
谭雄斯,庞武耀..HPLC波长切换法同时测定金嗓散结胶囊中的(R,S)-告依春、羟基红花黄色素A、丹酚酸B、木蝴蝶苷B和木蝴蝶苷A[J].中国现代中药,2017,19(12):1758-1761,1770,5.基金项目
肇庆市科技创新指导类项目[肇科(2017)33号文] （2017）
