安徽医科大学学报2017，Vol.52Issue(11)：1616-1622,7.DOI:10.19405/j.cnki.issn1000-1492.2017.11.008
携载BMP4基因的核壳结构纳米粒子修复兔桡骨临界骨缺损的研究
Bone regeneration in a critical sized defect using pBMP4-loaded TACS@EG-HBC particles
摘要
Abstract
Objective To evaluate the core-shell nanoparticles ecapsulated with bone morphogenetic protein 4 plasmids ability of bone regeneration in a critical sized radius defect.Methods 24 New Zealand white rabbits were randomly divided into two group:experimental groups and control group.Prepared 18 mm complete bone defect model at the bilateral radius,the experimental groups were implanted with TACS@EG-HBC/pBMP4/G and TACS/ pBMP4/G,While control groups implanted with only gelatin sponge.The radius bone defect were observed by bone mineral density and bone mineral content,anatomy,X ray,HE staining,immunohistochemistry and biomechanical testing at 2,4,8,12 weeks.Results All the 24 rabbits were in analysis and no injection and diapyesis was foundaround the incision.At 12 weeks after operation,new bone formation significantly increased in the TACS@EG-HBC/pBMP4/G treatment.It demonstrated significant increase in BMC and BMD values (P ＜ 0.05) compared to the other two groups at 2,4,8 and 12 weeks.X-ray also showed the reunion of bone marrow cavity at 12 weeks.HE staining:new bone trabecula interconnected turnedinto lamellar bone.Immunohistochemistry:protein BMP4staining with brown color was apparent.It also showed the bending stiffness of regenerated new bones at 12 weeks have no statistical differences compared with the normal radius.Conclusion TACS@EG-HBC/pBMP4/G is biosecurity and can be used to repair bone defect.关键词
壳聚糖/基因治疗/骨形态发生蛋白4/临界骨缺损/修复Key words
chitosan/gene therapy/bone morphogenetic protein 4/critical bone defects/repair分类
医药卫生引用本文复制引用
荣洁琳,林福星,汪谟贞,葛学武,赵宇..携载BMP4基因的核壳结构纳米粒子修复兔桡骨临界骨缺损的研究[J].安徽医科大学学报,2017,52(11):1616-1622,7.基金项目
国家自然科学基金(编号:81171829) （编号:81171829）
