安徽医科大学学报2017，Vol.52Issue(11)：1622-1626,5.DOI:10.19405/j.cnki.issn1000-1492.2017.11.009
PJA1及miR-130a基因在口腔肿瘤组织中的表达及相关性分析
Expression of PJA1 and miR-130a in oral tumor tissue and their correlation
摘要
Abstract
Objective To study the expression level of PJA1 and miR-130a in oral tumor tissue and to determine the effect on tumor cell migration and proliferation,and to explore the interaction between the two genes.Methods 15 groups of oral tumor and adjacent tissues were collected and their mRNA was extracted.The expression level of PJA1 and miR-130a was detected by qRT-PCR.Overexpression of PJA1,overexpression of miR-130a,overexpression of both PJA1 and miR-130a in SCC-3 were constructed.The effect of cell proliferation was examined by MTT assay,and the effect of cell migration was determined through Wound scratch assay.Results qRT-PCR revealed that the expression of PJA1 in oral tumor tissues was obviously lower than that in adjacent tissues (P ＜ 0.05),the expression of miR-130a in oral tumor tissues was significantly higher than that in adjacent tissues(P ＜ 0.05).MTT and Wound scratch assay demonstrated that overexpression of PJA1 could inhibit the proliferation and migration of SCC-3,overexpression of miR-130a could promote the proliferation and migration of SCC-3.PJA1 could inhibit the ability of miR-130a to promote the proliferation and migration of SCC-3 when both of them were overexpressed at the same time.Conclusion The expression level of PJA1 is down-regulated in oral tumor tissues,PJA1 can inhibit tumor cell proliferation and migration.The expression level of miR-130a is up-regulated in oral tumor tissues,miR-130a can promote tumor cell proliferation and migration.MiR-130a may promote oral tumor cell proliferation and migration by inhibiting PJA1.关键词
口腔肿瘤/PJA1/miR-130a/增殖/迁移Key words
oral tumor/PJA1/miR130a/proliferation/migration分类
医药卫生引用本文复制引用
许旭东,王银龙,朱友明,汪聪..PJA1及miR-130a基因在口腔肿瘤组织中的表达及相关性分析[J].安徽医科大学学报,2017,52(11):1622-1626,5.基金项目
国家自然科学基金(编号:31501103) （编号:31501103）
安徽省自然科学基金(编号:1508085QC62) （编号:1508085QC62）
