安徽医科大学学报2017，Vol.52Issue(12)：1819-1822,4.DOI:10.19405/j.cnki.issn1000-1492.2017.12.016
异丙酚预处理对大鼠肝脏缺血再灌注损伤时自噬的影响
Effect of propofol preconditioning on autophagy during hepatic ischemia reperfusion injury in rats
摘要
Abstract
Objective To evaluate the effect of propofol on autophagy during hepatic ischemia reperfusion in rats.Methods Fifty adult male Sprague-Dawely (SD) rats,weighing 240 ～280 g,were randomly divided into 5 groups (n =10 each)∶ sham operation group (group S);propofol alone group (group P);hepatic ischemia reperfusion (HIR) injury group(group HIR);propofol pretreatment group (group PHIR);intralipid pretreatment group (group IHIR).Propofol was infused at a rate of 12 mg/(kg · h) for 45 min in group P.HIR was induced by inflow occlusion to median and left liver lobes (approximately 70％) for 1 h of ischemia followed by 6 h reperfusion in male SD rats.Propofol was infused at a rate of 12 mg/(kg · h) 30 min before ischemia until 15 min of ischemia in group PHIR.Each group observed indicators∶ serum alanine aminotransferase (ALT),aspartate aminotransferase (AST);the expression of autophagy-related proteins such as microtubule associated protein light chain 3-Ⅰ (LC3-Ⅰ),microtubule associated protein light chain 3-Ⅱ (LC3-Ⅱ) and Beclin-1 was evaluated by Western blot.Results The electron microscopy showed that the structure of the hepatic cells was normal in group S,there were a lot of autophagosomes and autolysosomes in the hepatic cells in group HIR,and structure of the hepatic cells was normal in group PHIR.Compare with group S,the serum ALT and AST activity was significantly increased,the expression of LC3-Ⅱ/ LC3-Ⅰ and Beclin-1 was significantly up-regulated in group HIR(P ＜0.05),and no significant change was found in group P.Compared with group HIR,the ALT and AST activity was significantly decreased in serum,the expression of LC3-Ⅱ/LC3-Ⅰ and Beclin-1 was significantly down-regulated in group PHIR (P ＜ 0.05).No significant change was found in group IHIR.Conclusion Hepatic ischemia reperfusion injury could be alleviated by propofol preconditioning through suppression of autophagy in rats.关键词
异丙酚/肝脏/缺血再灌注损伤/自噬Key words
propofol/liver/ischemia reperfusion injury/autophagy分类
医药卫生引用本文复制引用
汤为香,沈玉君,李元海,沈玉先..异丙酚预处理对大鼠肝脏缺血再灌注损伤时自噬的影响[J].安徽医科大学学报,2017,52(12):1819-1822,4.基金项目
教育部高等学校博士学科点专项科研基金(编号:20133420110009) （编号:20133420110009）
