草业学报2017，Vol.26Issue(11)：47-56,10.DOI:10.11686/cyxb2017038
苜蓿DUS测试标准品种SSR分子标记指纹图谱的构建
Construction of SSR marker fingerprint database of standard alfalfa varieties utilizing DUS tests
摘要
Abstract
Due to cross-pollinating characteristic and the excessive use of backbone materials during the alfalfa breeding,the genetic diversity of alfalfa varieties is declining,and the discrimination of different varieties through phenotypical characteristics is becoming more difficult.The construction of a DNA fingerprint database of standard alfalfa varieties through the distinctness,uniformity and stability (DUS) test can facilitate the identification of alfalfa varieties.In this study we analyzed the genetic diversity among 33 standard alfalfa varieties with a DUS test and the DNA fingerprint of each variety was constructed based on 10 SSR markers.The results showed that a total of 69 alleles were generated from the 10 SSR markers,with an average of 6.9 alleles per marker;the polymorphic ratio varied from 25％ to 90％,with an average of 56.7％.The polymorphic information content per SSR marker varied from 0.56～0.87,with an average of 0.75.The 33 varieties could be clearly distinguished by the 10 SSR markers.The DNA fingerprint of each variety was established providing a technical basis for the validation and conservation of alfalfa varieties and genetic background analysis.关键词
苜蓿/SSR标记/指纹图谱/标准品种/DUS测试Key words
alfalfa/SSR markers/DNA fingerprinting/example varieties/DUS testing引用本文复制引用
闵学阳,刘文献,张正社,韦兴燚,齐晓,张义,刘志鹏,王彦荣..苜蓿DUS测试标准品种SSR分子标记指纹图谱的构建[J].草业学报,2017,26(11):47-56,10.基金项目
国家自然科学基金项目(31502000),教育部创新团队(IRT_17R50),高等学校学科创新引智计划(B12002),国家草品种区域试验项目(21301060001)和兰州大学中央高校基本科研业务费专项资金(lzujbky-2016-8)资助. （31502000）
