草业学报2017，Vol.26Issue(11)：57-68,12.DOI:10.11686/cyxb2017018
盐生草HgNHX1基因启动子的克隆及功能验证
Cloning and functional analysis of halophyte Halogeton glomeratus HgNHX1 promoter
摘要
Abstract
In order to test the function of pHgNHX1,the promoter sequence of the HgNHX1 gene from Halogeton glomeratus was cloned and a 1523 bp fragment flanking 5'-upstream of HgNHX1 was isolated and named pHgNHX1.The software PlantCARE and PLACE were used to predict and analyze the elements of pHgNHX1.The results showed that besides the TATA-box and CAAT-box,a number of potential cis-elements and transcription binding motifs related to stress responses were found,including salt,dehydration,cold and wound responsive elements.Other potential cis-elements responsive to phytohormones,including auxin,abscisic acid,gibberellin and ethylene were also found in the sequence.Sequence analysis indicated that it had the general characteristics of typical promoter.In order to evaluate the activity of pHgNHX1,we constructed pBI-pHgNHX1 expression vector and introduced it into tobacco and Arabidopsis using agrobacterium mediated transformation.The expression pattern was monitored using GUS histochemical staining.Results showed that GUS activity driven by the pHgNHX1 was detected in almost all vegetative and reproductive tissues,indicating that pHgNHX1 has a constitutive promoter activity.关键词
盐生草/HgNHX1基因/启动子/GUSKey words
Halogeton glomeratus/HgNHX1 gene/promoter/GUS引用本文复制引用
邹兰,王化俊,杨轲,徐先良,汪军成,任盼荣,姚立蓉,孟亚雄,李葆春,马小乐..盐生草HgNHX1基因启动子的克隆及功能验证[J].草业学报,2017,26(11):57-68,12.基金项目
973计划前期研究专项(2014CB160313),国家大麦青稞产业技术体系(CARS-05)和国家自然科学基金地区项目(31460347)资助. （2014CB160313）
