草业学报2017，Vol.26Issue(11)：69-76,8.DOI:10.11686/cyxb2017176
沟叶结缕草八氢番茄红素基因ZmPSY的克隆、亚细胞定位及表达分析
Cloning, subcellular localization and expression analysis of a novel phytoene synthase gene, ZmPSY, in Zoysia matrella
摘要
Abstract
The formation of phytoene by phytoene synthase is a key limiting step in the carotenoid biosynthesis pathway.In this study a novel gene,ZmPSY (GenBank Accession number:KY264128),was cloned from Zoysia matrella.Subcellular localization results proved that ZmPSY was localized in the chloroplasts.Expression analysis showed that ZmPSY had a higher expression level in leaves compared to roots and stems.The expression of ZmPSY in leaves also varied at different development stages,with the greatest expression seen in young leaves.qRT-PCR determination revealed that ZmPSY could be induced by exogenous abscisic acid (ABA),but suppressed by salicylic acid (SA) and methyl jasmonate (MeJA).This study paved the way for further discovery of the functional roles of ZmPSY.关键词
沟叶结缕草/八氢番茄红素基因/克隆/亚细胞定位/表达分析Key words
Zoysia matrella/phytoene synthase gene/cloning/subcellular localization/expression analysis引用本文复制引用
董笛,滕珂,于安东,檀鹏辉,梁小红,韩烈保..沟叶结缕草八氢番茄红素基因ZmPSY的克隆、亚细胞定位及表达分析[J].草业学报,2017,26(11):69-76,8.基金项目
北京市博后基金“苔草优良耐荫基因的挖掘与分子调控机制研究(No.2017-ZZ-087)”,北京市农林科学院博后基金,深圳市科技计划项目(JCYJ20160331151245672,JSGG20160229155434792)和国家高技术研究发展计划(863计划)(2013AA102607)资助. （No.2017-ZZ-087）
