福建农业学报2017，Vol.32Issue(9)：921-925,5.DOI:10.19303/j.issn.1008-0384.2017.09.001
水滴伪康纤虫全长cDNA文库的构建及EST分析
Full-length cDNA Library and EST Analysis on Pesudocohnilembus persalinus
摘要
Abstract
Pesudocohnilembus persalinus is a harmful parasite found in marine fish.Using SMART technology,a full-length cDNA library of P.persalinus was constructed.Titer of the primary library was 3.3 × 106 cfu · mL-1,and the library capacity was 1.5 × 107 cfu with a recombination rate of 97％.A PCR amplification on 24 random clones revealed that the inserted cDNA fragments ranged from 300-2 000 bp,with an average length over 500 bp.Subsequently,1 200 clones were selected from the library for EST sequencing.As a result,1 032 high-quality ESTs were obtained and assembled into 292 unigenes including 102 contigs and 190 unique ESTs with a redundancy rate of 71.7％.In a blast analysis against NCBI database,215 of the unigenes were homologous to the corresponding genes of ciliates with free-living and parasitic lifestyle,such as Tetrahyrnena therrnophila,Paramecium tetraurelia,Ichthyophthirius multi filiis,Cryptocaryon irritans,Oxytricha trifallax,etc.But,only 134 ESTs had been annotated.The remainders were hypothetical protein genes.The results obtained from this study would substantially facilitate the molecular cloning and characterization of the functional genes in P.persalinus.关键词
水滴伪康纤虫/cDNA文库/表达序列标签Key words
Pesudocohnilembus persalinus/cDNA library/EST sequencing分类
生物科学引用本文复制引用
林能锋,曾红..水滴伪康纤虫全长cDNA文库的构建及EST分析[J].福建农业学报,2017,32(9):921-925,5.基金项目
国家自然科学基金项目(31201698) （31201698）
福建省自然科学基金项目(2013J01120) （2013J01120）
福建省科技计划项目——省属公益类科研院所基本科研专项(2015R1019-11) （2015R1019-11）
