福建农业学报2017，Vol.32Issue(9)：926-931,6.DOI:10.19303/j.issn.1008-0384.2017.09.002
新-法二联灭活疫苗免疫不同日龄商品蛋鸡的效果
Immunological Effect on Egg-laying Chickens by Combining Vaccinations Against Newcastle and Infectious Bursal Diseases
摘要
Abstract
Immunological effect of combined inoculation of the inactivated vaccines for Newcastleand the infectious bursal diseases (ND-IBD) on egg-laying chickens was investigated.For the study,360 one-day-old commercial chicks were randomly divided into 3 groups.The chicks in one group were immunized immediately with the two vaccines simultaneously,another when they were 7-day-old,and the third group injected with saline solution as control.Levels of ND-IBD antibodies in the serum ofthese chicks were determined,spleen biopsies for disease detection executed,and qPCR on mRNA expressions of CD4+,CD8+,IL-6 and IFN-β performed every 7 days during the period of 21 to 56 d.The results showed that all treated one-or 7-day-old birds had higher antibody levels than control,and could fence off IBD virus infection.The 7-day-old group showed greater immunity against the diseases,and significantly increased mRNA expressions on CD4+,CD8+,IL-6 and IFN-β over control.It was,therefore,concluded that,by combining vaccinations against ND-IBD on 7-day-old egg-laying chicks,a desirable immunological effect on the chickens could be achieved.关键词
新城疫/传染性法氏囊/灭活苗/抗体检测Key words
newcastle disease/infectious bursal disease/inactivated vaccine/antibody detection分类
农业科技引用本文复制引用
星东,刘梦茜,袁晓琴,李春燕,陈仕怡,江兴华,赵华娥,包松英,王全溪..新-法二联灭活疫苗免疫不同日龄商品蛋鸡的效果[J].福建农业学报,2017,32(9):926-931,6.基金项目
国家星火计划重点项目(2015GA720001) （2015GA720001）
