福建农业学报2017，Vol.32Issue(9)：932-938,7.DOI:10.19303/j.issn.1008-0384.2017.09.003
鸡肠道大肠杆菌多抗菌株C20耐药性基因的预测和分析
Identification and Characterization of Antibiotic Resistance Genes from a Multi-drug Resistant Escherichia coli C20 Isolated from Guts of Chicken
摘要
Abstract
Spreading of antibiotic resistant bacteria in the environment has become a major concern for food safety and human health.Escherichia coli C20 was isolated from the gut microbiota of a domestic chicken that showed resistances to 7 out of 10 tested antibiotics.To elucidate the underlying mechanism,origin,and transmission of the resistances,published information on C20 genome were reviewed for a detailed analysis.There were 80 antibiotic resistance determinants,including the abovementioned 7 drugs,annotated in E.coli C20 genome from the databases.Among them,the fluoroquinolone-resistant genes were identified in the chromosomes,while those resistant to tetracycline,sulphonamide,chloramphenicol,and aminoglycoside found to be expressed in the plasmids of C20.A phylogenomic analysis on C20 revealed a clustering of the strain with E.coli K-12 group,especially the substrains of DH1,BW2952 and MG1655.Their genomic nucleoid sequences shared 97-99％ homology.There was no plasmid pC20-like sequence found in the genomic sequences of 3 K-12 strains reported previously.It was proposed that E.coli C20 isolated from the chicken guts might have acquired the antibiotic resistance through plasmid transfer from environmental microbiome.关键词
鸡肠道细菌/抗生素抗性/耐药性机制/基因组/水平基因转移Key words
chicken enteric bacteria/antibiotic resistance/drug resistance mechanism/genome/gene transfer分类
生物科学引用本文复制引用
田容川,黄薇..鸡肠道大肠杆菌多抗菌株C20耐药性基因的预测和分析[J].福建农业学报,2017,32(9):932-938,7.基金项目
福建省自然科学基金项目(2014J01111、2017J01625) （2014J01111、2017J01625）
