福建农业学报2017，Vol.32Issue(9)：939-945,7.DOI:10.19303/j.issn.1008-0384.2017.09.004
杂交水稻恢复系福恢7185 OsSDR1基因的克隆与序列分析
Cloning and Sequence Analysis of OsSDR1 Gene from Restorer Line of Hybrid Rice Fuihui 7185
摘要
Abstract
The transition from dormancy to germination in seeds is a key physiological process during the lifecycle of plants.Hormones play an important regulatory role on it,While,short-chain dehydrogenase/reductase (SDR1) is a molecular link between nutrient signaling and plant hormone biosynthesis.The coding sequences (CDS) of SDR1 were cloned from different dormancy varieties FH7185,FH653 and YH290,respectively.The results of sequences blast indicated that the coding regions of OsSDR1 among the three varieties were homologous,but there were 9 bases deletion,coding three alanine compared to Nipponbare.Meanwhile,the results of real-time quantitative PCR (qRT-PCR) of SDR1 expression level in seed embryo among different dormancy varieties indicated that the expression level of SDR1 was significantly different among them.Moreover,the corresponding interaction proteins of OsSDR1 were screened by GST pull-down experiments and an interaction protein of OsSDR1 was found and its Genbank serial number designated as Os05g0140800,it was verified related to seed dormancy.The preliminary results showed that SDR1 gene might regulate seed dormancy through interaction with other structural elements.关键词
水稻/福恢7185/OsSDR1/克隆/序列分析/互作蛋白Key words
rice/Fuhui 7185/OsSDR1/cloning/sequence analysis/interaction protein分类
农业科技引用本文复制引用
许惠滨,谢华安,张建福,徐倩,连玲,朱永生,蒋家焕,谢鸿光,罗曦,江敏榕,郑燕梅..杂交水稻恢复系福恢7185 OsSDR1基因的克隆与序列分析[J].福建农业学报,2017,32(9):939-945,7.基金项目
国家自然科学基金项目(31501387) （31501387）
福建省自然科学基金项目(2015J05060) （2015J05060）
杂交水稻国家重点实验室开放课题(2016KF11) （2016KF11）
福建省农业科学院青年英才计划项目(YC2015-14) （YC2015-14）
