福建农业学报2017，Vol.32Issue(9)：946-950,5.DOI:10.19303/j.issn.1008-0384.2017.09.005
彩色马铃薯茎尖培养及病毒检测技术
Stem-tip Tissue Culture and Virus Detection on Plantlets of Color Potato
摘要
Abstract
Stem-tips of potato (Solanum tuberosum L.) plants that bear colored tubers were used to produce virusfree plantlets in test tubes.Culture media with hormone combinations in varying concentrations were tested to determine their effects on 21 varieties of plantlets offspring of the potato tissue culture.Meanwhile,RT-PCR was employed to detect any viruses present in the plantlets.The optimum medium for propagating a chosen cultivar,Minshu No.1,was formulated on MS with 0.05 mg · L-1 NAA,0.1 mg · L-1 6-BA,0.2 mg · L-1 GA3 and 0.2 mg · L-1 calcium pantothenate.Different hormone combinations led to significantly different stem-tip differentiation and plantlet formation among the potato varieties.A total of 11 test tube cultivars derived from the stem-tip cultures were virus-free without the contamination of PVX,PVS,PVA,PVS,PVM or PSTVd.They could be the candidate plantlets for field planting.关键词
彩色马铃薯/茎尖脱毒/RT-PCR/病毒检测Key words
color potato/virus-free stem-tip/RT-PCR/virus detection分类
农业科技引用本文复制引用
许泳清,邱思鑫,汤浩,李华伟,纪荣昌,罗文彬,李国良,刘中华,张鸿,林赵淼,邱永祥..彩色马铃薯茎尖培养及病毒检测技术[J].福建农业学报,2017,32(9):946-950,5.基金项目
福建省科技计划项目——省属公益类科研院所基本科研专项(2015R1026-11) （2015R1026-11）
