福建农业学报2017,Vol.32Issue(9):955-958,4.DOI:10.19303/j.issn.1008-0384.2017.09.007
辣木转录组SSR位点信息分析
SSR Loci in Transcriptome of Moringa oleifera Lam
摘要
Abstract
MISA software was employed to analyze the distribution and basic characteristics ot SSR loci of 15 824 unigenes,which were longer than 1 kb,from the transcriptome of Moringa oleifera Lam leaves at different development stages.A total of 8 272 SSR loci consisting of 114 motifs were detected in 6 003 unigenes,i.e.,a frequency of 52.28%.The most abundant repeating motif was a single nucleotide (55.69%),which was followed by dinucleotide (32.34%) and trinucleotide (11.24%);and,their SSRs were A/T,AG/CT and AAG/CTT,respectively.The most commonly found SSRs had 10-20 tandem repeats (30.35%) with their motif lengths ranging from 12 bp to 19 bp.It appeared that there were numerous SSRs in the transcriptomes with high distribution frequencies and many varieties.The results obtained from this study provided valuable information for developing SSR markers.关键词
辣木/转录组/SSRKey words
Moringa oleifera/transcriptome/SSR分类
农业科技引用本文复制引用
张雅玲,韦晓霞,王小安,潘少霖,叶新福..辣木转录组SSR位点信息分析[J].福建农业学报,2017,32(9):955-958,4.基金项目
福建省科技计划项目——省属公益类科研院所基本科研专项(2016R1013-8) (2016R1013-8)
福建省区域发展项目(2016N3017) (2016N3017)