福建农业学报2017，Vol.32Issue(9)：959-962,4.DOI:10.19303/j.issn.1008-0384.2017.09.008
秋石斛原球茎液体增殖培养研究
The Study on Dendrobium spp.PLB Propagation in Liquid Culture
摘要
Abstract
The protocorm-like body (PLB) induced from stem tips of Dendrobium spp.Sanya Sunny'were used as explants,The orthogonal design and the liquid media were used to study the effects of the key factors such as culture medium,TDZ,eoconut water and sugar contained on PLB propagation of Dendrobium spp.‘Sanya Sunny’.Through above research,it was tried to find the main factors and build up the PLB proliferation the system in Dendrobium spp.Sanya Sunny'in the paper.The results showed that the main factors of PLB propagation are the basic medium,TDZ,sugar and coconut water.The best multiplication medium of PLB is improvement HM # 2 medium,TDZ 0.5 mg · L-1,NAA 0.1 mg · L-1,coconut water 20.0 mL · L-1,and sugar 30.0 g L-1 to result in an averaged propagation coefficient of 6.25 in 35 days.关键词
秋石斛/原球茎/增殖/正交设计/液体培养Key words
Dendrobium spp./protocorm-like body/propagation/Orthogonal Design/liquid culture分类
农业科技引用本文复制引用
叶秀仙,林榕燕,黄敏玲,钟淮钦,林兵..秋石斛原球茎液体增殖培养研究[J].福建农业学报,2017,32(9):959-962,4.基金项目
福建省科技计划项目——省属公益科研院所基本科研专项(2015R1026-8) （2015R1026-8）
福建省财政专项——福建省农科院科技创新团队PI项目(2016PI-39) （2016PI-39）
福建省农业科学院导师制基金项目(2014QA-5) （2014QA-5）
